JCRB1999 m5S-RFP

Cell information

Important Notice(s)

The cultivation and live cell imaging of m5S-RFP/JCRB1999 m5S-RFPの培養とライブセルイメージング

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1999 Cell Name   m5S-RFP
Profile   Nuclei of m5S cells, near-diploid mouse embryonic cell line, visualized with mCherry-histone H3. Other Name   
Animal   mouse Strain   ICR
Genus   Mus Species   musculus
Sex   M Age   17.5-day p.c.
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Near-diploid chromosome number, contact inhibition, non-tumorigenic.　Karyotype: same as the parental m5S cells except for losing one of chromosome 13.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Nuclei of m5S cells,near-diploid mouse embryonic cell line, visualized with mCherry-histone H3.
Classify   normal Established by   Kenji SUGIMOTO
Registered by   Kenji SUGIMOTO Regulation for Distribution   
Comment   Used vector is mCherry-histone H3 plasmid DNA. Year   
Medium   DMEM (4.5 g/L glucose) supplemented with 10% fetal bovine serum Methods for Passages   0.25% trypsin and 0.02% EDTA in PBS(-)
Cell Number on Passage   Sprit ratio of about 1/10 Race   
CO2 Conc.   5% Tissue Sampling   Skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:33077361Pseudo-nuclear staining of cells by deep learning improves the accuracy of automated cell counting in a label-free cellular population.
Tsuzuki Y,Sanami S,Sugimoto K,Fujita S
J Biosci Bioeng. 2021 Feb;131(2):213-218

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1999 Cell Name   m5S-RFP
LOT No.   02092026 Lot Specification   distribution
Medium   DMEM (4.5g/L D-Glucose, GIBCO Cat#11995-065) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K). Temperature   37 C
Cell Density at Seeding   4.95-6.16x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   8.18x10^5
Viability at cell freezing (%)   96.77 Antibiotics Used   free
Passage Number   p5 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   100 Additional information   Ampoules were printed JCRB4116 by mistake. JCRB1999 is correct.
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