JCRB1205 HCSC-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1205 | Cell Name | HCSC-1 |
|---|---|---|---|
| Profile | Human endocervical carcinoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 48 |
| Identity | available | Tissue for Primary Cancer | uterine cervix |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | small cell carcinoma | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | small cell carcinoma |
| Classify | tumor | Established by | Shimoda,T. & Kuramoto, H. |
| Registered by | Kuramoto, H. | Regulation for Distribution | |
| Comment | Year | 2005 | |
| Medium | Eagle's MEM with 10 % fetal calf serum. | Methods for Passages | 0.1% trypsin and 0.02% EDTA |
| Cell Number on Passage | 10^4 cells/60mm dish | Race | Japanese |
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type | small cell carcinoma of the uterine cervix |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | + | ||||||
| Notes | |||||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1205 | Cell Name | HCSC-1 |
|---|---|---|---|
| LOT No. | 08112007 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% heat inactivated fetal bovine serum(Intergen RB52305) | Temperature | 37 C |
| Cell Density at Seeding | 3.1x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.1x10^6 |
| Viability at cell freezing (%) | 94.5 | Antibiotics Used | free |
| Passage Number | p302 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:8,9 D7S820:11,12 D16S539:9,13 VWA:16,18 TH01:9 AM:X TPOX:9 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Culture medium with 5% DMSO | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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| Cell No. | JCRB1205 | Cell Name | HCSC-1 |
|---|---|---|---|
| LOT No. | 10042024 | Lot Specification | distribution |
| Medium | Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37C |
| Cell Density at Seeding | 2.1 - 2.9 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 53 hrs. | Cell Number in Vial (cells/1ml) | 5.1 x 10^6 |
| Viability at cell freezing (%) | 86 | Antibiotics Used | free |
| Passage Number | P305 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:8,9 D7S820:11,12 D16S539:9,13 VWA:16,18 TH01:9 AM:X TPOX:9 CSF1PO:11,12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - EMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 83 | Additional information |