JCRB1153 HCA-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1153 Cell Name   HCA-1
Profile   Human endocervical adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   60
Identity   available Tissue for Primary Cancer   uterine cervix
Case history    Metastasis   No
Tissue Metastasized    Genetics   endocervical adenocarcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endocervical adenocarcinoma
Classify   tumor Established by   Shimoda,T. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   Eagle's minimum essential medium with 10% calf serum. (Current medium for propagation is Eagle's minimum essential medium with 10% FBS.) Methods for Passages   Cell are harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/ml Race   Japanese
CO2 Conc.   5 % Tissue Sampling   lymph node metastasis
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2916
Reference
Pubmed id:noneHCA-1
Shimoda T, Kuramoto H
Human Cell. 1988 1:455

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1153 Cell Name   HCA-1
LOT No.   10122010 Lot Specification   distribution
Medium   MEM(GIBCO) with 10% heat inactivated fetal bovine serum (SIGMA Cat. # F0926, Lot 027K03911) Temperature   37 C
Cell Density at Seeding   2.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.65x10^6
Viability at cell freezing (%)   96.9 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:10,11
D16S539:11
VWA:18
TH01:7
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1153 Cell Name   HCA-1
LOT No.   04252006 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum. Temperature   37 C
Cell Density at Seeding   2.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1x10^6
Viability at cell freezing (%)   88.1 Antibiotics Used   Free
Passage Number   P2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined.Human,not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:10,11
D16S539:11
VWA:18
TH01:7
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   Ampules are mislabeled as HCA. The correct name is HCA-1 as shown in the data-sheet.
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