JCRB1152 RSMG-2

Cell information

Important Notice(s)

Use and cultivation of RSMG-1 and RSMG-2 cell lines

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1152 Cell Name   RSMG-2
Profile   Stem cell like cell line from male rat submandibular gland. Other Name   
Animal   rat Strain   Wistar
Genus   Rattus Species   norvegicus
Sex   M Age   10-week-old
Identity    Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Growth of the RSMG-2 cells are inhibited by activin A, while accelarated by follistatin. The RSMG-2 cells shows a stem cell like characteristics.
Classify   normal Established by   Furue,M.
Registered by   Furue,M. Regulation for Distribution   Must cite the references, {5541,5544}.
Comment   Co-operative research with Dr. Furue,M. recommended because of the special characteristics of the RSMG cells. Year   2004
Medium   Serum free MCDB153 medium (Research Inst. for Functional Peptides, Yamagata, Japan). Methods for Passages   Cells are harvested after treatment with 0.05% trypsin in 0.04% EDTA then inactivated by 0.1% soybean trypain inhibitor.
Cell Number on Passage   split ratio = 1/4 Race   
CO2 Conc.   5 % Tissue Sampling   selection from whole body culture
Tissue Type   submandibular gland epithelial cells

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8311
Reference
Pubmed id:11401526Activin A induces expression of rat Sel-1l mRNA, a negative regulator of notch signaling, in rat salivary gland-derived epithelial cells.
Furue M,Zhang Y,Okamoto T,Hata RI,Asashima M
Biochem Biophys Res Commun. 2001 Apr 6;282(3):745-9
Pubmed id:10937830Isoleucine prevents rat salivary gland epithelial cells from apoptosis in serum-free culture.
Furue M,Okamoto T,Koshika S,Asashima M
In Vitro Cell Dev Biol Anim. 2000 May;36(5):287-9
Pubmed id:10476908Effects of hepatocyte growth factor (HGF) and activin A on the morphogenesis of rat submandibular gland-derived epithelial cells in serum-free collagen gel culture.
Furue M,Okamoto T,Hayashi H,Sato JD,Asashima M,Saito S
In Vitro Cell Dev Biol Anim. 1999 Mar;35(3):131-5
Pubmed id:9719409Hepatocyte growth factor regulates activin betaA mRNA in submandibular gland.
Furue M,Saito S
In Vitro Cell Dev Biol Anim. 1998 Jul-Aug;34(7):520-3

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1152 Cell Name   RSMG-2
LOT No.   08072008 Lot Specification   distribution
Medium   MCDB153+5F+F Temperature   37 C
Cell Density at Seeding   1.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin(GIBCO) and 0.04% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.3x10^6
Viability at cell freezing (%)   89.7 Antibiotics Used   NT
Passage Number   p26 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   MCDB153+5F+F+FAF-BSA with 10% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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