JCRB1145 HEC-50B

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1145 Cell Name   HEC-50B
Profile   Human endometrioid adenocarcinoma (G3) from japanese patient. Other Name   HEC-50
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Hamano, M. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   Eagle's minimal essential medium with 15% fetal calf serum. Methods for Passages   Harvested with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   peritoneal fluid
Tissue Type   endometrioid adenoca. (uterine corpus)

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2929
Reference
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60
Pubmed id:2064198Establishment and characterization of human endometrial cancer cell lines.
Kuramoto H,Nishida M,Morisawa T,Hamano M,Hata H,Kato Y,Ohno E,Iida T
Ann N Y Acad Sci. 1991;622():402-21
Pubmed id:269609Cytogenetic studies of human endometrial carcinomas by means of tissue culture.
Kuramoto H,Hamano M
Acta Cytol. 1977 Jul-Aug;21(4):559-65

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1145 Cell Name   HEC-50B
LOT No.   03032006 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   3.1x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   92.7 Antibiotics Used   Free
Passage Number   p859 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8
D13S317:9
D7S820:12
D16S539:12
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1145 Cell Name   HEC-50B
LOT No.   10182013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10099). Temperature   37 C
Cell Density at Seeding   3.6 - 5.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   4.7 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P862 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8
D13S317:9
D7S820:12
D16S539:12
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,12
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   91.0 Additional information   
Cell No.   JCRB1145 Cell Name   HEC-50B
LOT No.   07232024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.9 - 1.0 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 41 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   98.1 Antibiotics Used   free
Passage Number   P862 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:8
D13S317:9
D7S820:12
D16S539:12
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   90.6 Additional information   
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