JCRB1142 HEC-265

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1142 Cell Name   HEC-265
Profile   Human endometrioid adenocarcinoma, G1 from uterus. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Fujisawa,T. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   free
Comment    Year   2005
Medium   Eagle's minimal essential medium with 15% fetal calf serum. Methods for Passages   Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA.
Cell Number on Passage   1-2 x10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2928
Reference
Pubmed id:15171304Establishment and characterization of two different types of new human endometrial adenocarcinoma cell lines (HEC-251 and HEC-265).
Fujisawa T,Hamano M,Hata H,Kamata Y,Watanabe J,Sekimoto R,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(3):299-304
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60


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Pubmed id:none ヒト子宮類内膜がん細胞株表面における免疫チェックポイント分子PD-L1の発現調節に果たすEzrin/Radixin/Moesinの役割
小堀 宅郎, 田中 千都, 伊藤 優衣, 浦嶋 庸子, 伊藤 卓也, 高垣 伸匡, 小畑 友紀雄
Journal of AYA Oncology Alliance　Vol.3 No.1 2023

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1142 Cell Name   HEC-265
LOT No.   02072006 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   2.9x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.7x10^6
Viability at cell freezing (%)   91.4 Antibiotics Used   Free
Passage Number   P187 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:11,12
D7S820:10,11
D16S539:11
VWA:19,22
TH01:9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1142 Cell Name   HEC-265
LOT No.   06132013 Lot Specification   distribution
Medium   Eagle's minimal essential medium with 15% fetal calf serum. (FBS; GIBCO Cat. No. #10099) Temperature   37C
Cell Density at Seeding   0.6-1.3 x10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypisn and 0.02% EDTA.
Doubling Time    Cell Number in Vial (cells/1ml)   3.3 x 10^6 cells/1mL
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P189 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB),NP,LDH were examined. Human,not HeLa
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:11,12,13
D7S820:10,11
D16S539:11
VWA:19,21,22,23
TH01:9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion    Exoteric Gene   
Medium for Freezing   Culture medium with 10% DMSO CO2 Conc.   5%
Viability immediately after thawing (%)   94.4 Additional information   
Cell No.   JCRB1142 Cell Name   HEC-265
LOT No.   12152017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012). Temperature   37 C
Cell Density at Seeding   1-2 x10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypisn.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   85 Antibiotics Used   free
Passage Number   P189 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   85 Additional information   
Cell No.   JCRB1142 Cell Name   HEC-265
LOT No.   08062020 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   7 - 9 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 30 hrs. Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   approx. 10 Antibiotics Used   free
Passage Number   P189 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   5% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
Cell No.   JCRB1142 Cell Name   HEC-265
LOT No.   09192023 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 107322) Temperature   37 C
Cell Density at Seeding   1.4 - 1.7 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 25-28 hrs. Cell Number in Vial (cells/1ml)   2.5 x 10^6
Viability at cell freezing (%)   88.7 Antibiotics Used   free
Passage Number   P190 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,12
D13S317:11,12
D7S820:10,11
D16S539:11
VWA:19,22
TH01:9
AM:X
TPOX:9,11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   91.6 Additional information   
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