JCRB1141 HEC-251

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1141 Cell Name   HEC-251
Profile   Human endometrial carcinoma from uterus body. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   uterus
Case history    Metastasis   No
Tissue Metastasized    Genetics   endometrial carcinoma
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   endometrial carcinoma
Classify   tumor Established by   Fujisawa,T. & Kuramoto, H.
Registered by   Kuramoto, H. Regulation for Distribution   
Comment    Year   2005
Medium   Eagle's minimal essential medium with 15% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 x 10^5 cells/mL Race   Japanese
CO2 Conc.   5 % Tissue Sampling   uterine corpus
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2927
Reference
Pubmed id:15171304Establishment and characterization of two different types of new human endometrial adenocarcinoma cell lines (HEC-251 and HEC-265).
Fujisawa T,Hamano M,Hata H,Kamata Y,Watanabe J,Sekimoto R,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(3):299-304
Pubmed id:15053063Quantitative study on the correlation between p53 gene mutation and its expression in endometrial carcinoma cell lines.
Kamata Y,Watanabe J,Hata H,Hamano M,Kuramoto H
Eur J Gynaecol Oncol. 2004;25(1):55-60

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1141 Cell Name   HEC-251
LOT No.   08062010 Lot Specification   distribution2
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA Cat.#F0926 Lot 027K03911) Temperature   37 C
Cell Density at Seeding   0.38-1.64x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.45x10^6
Viability at cell freezing (%)   89.2 Antibiotics Used   free
Passage Number   p205 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,12
D13S317:11
D7S820:11
D16S539:9
VWA:14
TH01:7
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)   77.3 Additional information   
Images
Cell No.   JCRB1141 Cell Name   HEC-251
LOT No.   07212010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   3.87-4.52x10^2cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.12x10^6
Viability at cell freezing (%)   91.5 Antibiotics Used   free
Passage Number   p202 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,12
D13S317:11
D7S820:11
D16S539:9
VWA:14
TH01:7
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1141 Cell Name   
LOT No.   06222010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (GIBCO Cat. # 10099) Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/mL Methods for Passages   0.1% trypsin and 0.02% EDTA in Ca, Mg-free PBS.
Doubling Time   approx. 44 hrs Cell Number in Vial (cells/1ml)   3.3 x 10^6
Viability at cell freezing (%)   79.3 Antibiotics Used   free
Passage Number   P203 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB1141 Cell Name   HEC-251
LOT No.   12222005 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum Temperature   37 C
Cell Density at Seeding   1.3x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   Free
Passage Number   p200 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,12
D13S317:11
D7S820:11
D16S539:9
VWA:14
TH01:7
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1141 Cell Name   HEC-251
LOT No.   07082019 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   5.8 - 6.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 65 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   98.7 Antibiotics Used   free
Passage Number   P204 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   91.4 Additional information   
Cell No.   JCRB1141 Cell Name   HEC-251
LOT No.   06242024 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37C
Cell Density at Seeding   2 - 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin.
Doubling Time   approx. 61 hrs. Cell Number in Vial (cells/1ml)   1.8 x 10^6
Viability at cell freezing (%)   94.3 Antibiotics Used   free
Passage Number   P206 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:9,12
D13S317:11
D7S820:11
D16S539:9
VWA:14
TH01:7
AM:X
TPOX:8,11
CSF1PO:9,11
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   84.9 Additional information   
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