JCRB1606 Onda 10

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1606 Cell Name   Onda 10
Profile   Human cell line derived from human glioma. Other Name   no.10
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62-year-old
Identity   available Tissue for Primary Cancer   brain
Case history   anaplastic glioma Metastasis   No
Tissue Metastasized    Genetics   P53 mutation, PTEN expression (-)
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   -
Classify   tumor Established by   Onda, K.
Registered by   Kumanishi, T. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   0.02% Trypsin + 0.01% EDTA
Cell Number on Passage   1:3 - 1:5 /week Race   Japanese
CO2 Conc.    Tissue Sampling   brain
Tissue Type   anaplastic glioma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3075
Reference
Pubmed id:3188836Correlation of DNA ploidy and morphological features of human glioma cell cultures with the establishment of cell lines.
Onda K,Tanaka R,Washiyama K,Takeda N,Kumanishi T
Acta Neuropathol. 1988;76(5):433-40
Pubmed id:noneGFAP-positive human glioma cell lines: no.11, no.10.
Onda K., Washiyama K., Tanaka R., Nishiyama A., Usui H., Kumanishi T.
Human Cell 3:251-252(1990)

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1606 Cell Name   Onda 10
LOT No.   09122014 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.83-4.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.02% trypsin and 0.01% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.05x10^6
Viability at cell freezing (%)   96.6 Antibiotics Used   free
Passage Number   p63 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:12
D7S820:8,11
D16S539:11
VWA:17
TH01:7,9
AM:X,Y
TPOX:9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1606 Cell Name   Onda 10
LOT No.   11272018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.0 - 4.3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 43 hrs. Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   P66 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   87.4 Additional information   
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