JCRB1342.09 MOR158.1.3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1342.09 Cell Name   MOR158.1.3
Profile   Mouse hybridoma cell line producing monoclonal antibody against morphine. Other Name   
Animal   mouse x mouse Strain   
Genus   hybridoma Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   Producing IgG1 k.
Classify   hybridoma Established by   Sawada, J.
Registered by   Sawada, J. Regulation for Distribution   
Comment    Year   2007
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   dilution
Cell Number on Passage   split 1/5 (1/2 - 1/10) Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   producing Mab morphine.

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J472
Reference
Pubmed id:8417377Molecular and biochemical analyses of combining sites of monoclonal anti-morphine antibodies.
Sawada J,Yamazaki T,Terao T
Mol Immunol. 1993 Jan;30(1):77-86
Pubmed id:3211162Production and characterization of high-affinity monoclonal antibodies against morphine.
Sawada J,Janejai N,Nagamatsu K,Terao T
Mol Immunol. 1988 Sep;25(9):937-43

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1342.09 Cell Name   MOR158.1.3
LOT No.   10162009 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(Hyclone GRD0051) Temperature   37 C
Cell Density at Seeding   0.3-1.3x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.34x10^6
Viability at cell freezing (%)   93.8 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1342.09 Cell Name   MOR158.1.3
LOT No.   01222019 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% heat-inactivated fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.7 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture). The size of cells is small. At the time of centrifugation, 1200 rpm will be adequate.
Doubling Time   approx. 25 hrs. Cell Number in Vial (cells/1ml)   5.0 x 10^5
Viability at cell freezing (%)    Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   64.1 Additional information   
CLOSE