JCRB1058 XP40OSTERT

Cell information

Important Notice(s)

XP（色素性乾皮症患者）由来細胞

Cell type：human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB1058 Cell Name   XP40OSTERT
Profile   hTERT transformed fibroblast cell line from Xeroderma pigmentosum. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   skin
Case history   Xeroderma pigmentosum Group C. Metastasis   
Tissue Metastasized    Genetics   Xeroderma pigmentosum (group C), fibroblast, transformed with hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   hTERT transformed Xeroderma pigmentosum cell line.
Classify   transformed Established by   Tachibana,A.
Registered by   Tachibana,A. Regulation for Distribution   
Comment    Year   2002
Medium   alpha-MEM medium with 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with trypsin and EDTA.
Cell Number on Passage   split ratio = 1/4 Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   hTERT transformed cells

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3251
Reference
Pubmed id:7155047Kinetic analysis of polyethylene glycol-induced cell fusion in cultured human fibroblasts: its application to genetic complementation analysis of xeroderma pigmentosum.
Sato K,Ikenaga M,Sano S
Med J Osaka Univ. 1982 Sep;33(1-2):19-28

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1058 Cell Name   XP40OSTERT
LOT No.   10142003 Lot Specification   distribution
Medium   Alpha-MEM medium 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin. Subculture once 4 - 7 days. Medium change every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.8 x 10^5
Viability at cell freezing (%)   89.8 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1058 Cell Name   XP40OSTERT
LOT No.   10152003 Lot Specification   distribution
Medium   Alpha-MEM medium with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   1.0 x 10^4 cells/sq.cm. Methods for Passages   Cells are harvested after treatment with 0.25% trypsin. Subculture every 4-7 days and medium change every 3-4 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.7 x 10^5
Viability at cell freezing (%)   92.6 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD(typeB), NP, LDH examined. Human not HeLa.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
Images
Cell No.   JCRB1058 Cell Name   XP40OSTERT
LOT No.   08282003 Lot Specification   distribution
Medium   Alpha-MEM with 10% fetal calf serum (Intergen RB52305). Temperature   37 C
Cell Density at Seeding   0.5-1 x 10^4 cells/sq.cm. Methods for Passages   Cells were harvested after treatment with 0.25% trypsin. Subculture once a week and medium change every 3 days.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   98.9 Antibiotics Used   free
Passage Number   P4* PDL   NT
Sterility: MYCOPLASMA   NT Sterility: BACTERIA   NT
Sterility: FUNGI   + Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium with 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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