-Detailed Information [JCRB0828]-

JCRB0828 HO-1-u-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0828	Cell Name	HO-1-u-1
Profile	squamous carcinoma, mouth floor	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	72
Identity	available	Tissue for Primary Cancer	oral
Case history		Metastasis
Tissue Metastasized		Genetics
Life Span		Crisis PDL
Morphology		Character	squamous cell carcinoma
Classify		Established by	Miyauchi,S. & Takada,K.
Registered by	Akiyama,M	Regulation for Distribution
Comment		Year	1988
Medium	Dulbecco's modified Eagle's medium - F12 medium (1:1 mix) with 10% fetal calf serum.	Methods for Passages	Cells are treated with 0.1 % trypsin and 0.02 % EDTA. Dilute in fresh medium.
Cell Number on Passage	split ratio=1/6	Race
CO2 Conc.	5 %	Tissue Sampling	mouth floor
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_2784
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	753561

Reference
Pubmed id:2997498	[An autopsy case of triple cancer associated with superior vena cava syndrome]. Sugiyama A,Ishiguro M,Yasue T,Watanabe S,Sugishita N,Yokoyama H,Uno T,Watanabe H,Matsuno Y,Sasaoka I Gan No Rinsho. 1985 Aug;31(10):1347-52

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

111988
080995
090495
10172000
05262014

Cell No.	JCRB0828	Cell Name	HO-1-u-1
LOT No.	111988	Lot Specification	distribution
Medium	DM-F12 + 10 % FBS	Temperature	37
Cell Density at Seeding	1:6	Methods for Passages	0.1 % trypsin + 0.02 % EDTA
Doubling Time	NT	Cell Number in Vial (cells/1ml)	1-2x10^6
Viability at cell freezing (%)	95%	Antibiotics Used	P/S
Passage Number	*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0828	Cell Name
LOT No.	080995	Lot Specification	distribution
Medium	DM-F12 + 10 % FBS	Temperature	37
Cell Density at Seeding	1:6	Methods for Passages	0.1 % trypsin + 0.02 % EDTA
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0828	Cell Name
LOT No.	090495	Lot Specification	distribution
Medium	DM-F12 + 10 % FBS	Temperature	37
Cell Density at Seeding	1:6	Methods for Passages	0.1 % trypsin + 0.02 % EDTA
Doubling Time		Cell Number in Vial (cells/1ml)
Viability at cell freezing (%)		Antibiotics Used
Passage Number		PDL
Sterility: MYCOPLASMA		Sterility: BACTERIA
Sterility: FUNGI		Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0828	Cell Name
LOT No.	10172000	Lot Specification	distribution
Medium	Dulbecco's modified Eagle's medium : Ham's F12 medium = 1:1 with10% fetal calf serum	Temperature	37 C
Cell Density at Seeding		Methods for Passages	0.1% trypsin and 0.02% EDTA
Doubling Time		Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	94	Antibiotics Used	free
Passage Number	P83	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0828	Cell Name	HO-1-u-1
LOT No.	05262014	Lot Specification	distribution
Medium	1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	2.2 - 6.2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	3.6 x 10^6
Viability at cell freezing (%)	62.1	Antibiotics Used	free
Passage Number	P84	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:10 D13S317:9 D7S820:9,10 D16S539:12 VWA:17,19 TH01:9 AM:X,Y TPOX:10 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	62.1	Additional information	Lot# is labeled as 052620104.