JCRB0544 TIG-3S

Cell information

Important Notice(s)

Notes for the cultivation of human normal diploid cells (JCRB05**) : ヒト正常二倍体細胞の取り扱い上の注意点

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0544 Cell Name   TIG-3S
Profile   Normal diploid fibroblast from human male skin with limited life span. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   fetus
Identity   not done Tissue for Primary Cancer   skin
Case history   normal Metastasis   
Tissue Metastasized    Genetics   2n=46, XY
Life Span   finite Crisis PDL   PD67
Morphology   fibroblast-like Character   lifespan PD67
Classify   normal Established by   TIG
Registered by   Kimura,S. & Kondo,H. Regulation for Distribution   
Comment    Year   2004
Medium   Eagle's minimal essential medium with 10% fetal bovine serum. Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5 % Tissue Sampling   skin
Tissue Type   normal diploid fibroblast

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_3210
Reference
Pubmed id:7556460Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.
Kondo H,Yonezawa Y
Exp Cell Res. 1995 Oct;220(2):501-4




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0544 Cell Name   TIG-3S
LOT No.   11042010 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (GIBCO Cat. # 10099).This lot was frozen using DMSO. Temperature   37 C
Cell Density at Seeding   6 - 7 x 10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 35 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   31
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10,13
D13S317:9,12
D7S820:10,12
D16S539:10
VWA:16,17
TH01:7,9
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   JCRB0544 Cell Name   TIG-3S
LOT No.   05152025 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.6 - 1.2 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 28 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   88.8 Antibiotics Used   free
Passage Number   Unknown (3 at bank) PDL   29
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
Viability immediately after thawing (%)   76.6 Additional information   
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