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Rat embryo cell line with ts mutation for cell cycle progression from G1 to S phase.
Other Name
tsJT663(3Y1)
Animal
rat
Strain
Fischer 344
Genus
Rattus
Species
norvegicus
Sex
F
Age
fetus
Identity
Tissue for Primary Cancer
embryo
Case history
normal
Metastasis
Tissue Metastasized
Genetics
A ts mutant for cell cycle progression from G1 to S phase.
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
MNNG-mutagenized 3Y1 cell derivatives with ts mutation grows at 34 C but the growth is arrested at 40 C.
Classify
mutant
Established by
Nihomiya-Tsuji,J. and Ide.T.
Registered by
Kyoizumi,S.
Regulation for Distribution
free
Comment
Year
2000
Medium
Dulbecco's modified Eagle's medium with 0.1 % glucose and 10% fetal calf serum.
Methods for Passages
Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. Gentle piptting may be reauired to detach cells from dishes. Subculture once a week at split ratio=1/8 at 34 C.
Cell Number on Passage
5-8 x 10^5 cells/60mm dish
Race
CO2 Conc.
5 %
Tissue Sampling
embryo
Tissue Type
Detection of virus genome fragment by Real-time PCR
Isolation of a mammalian cell cycle ts mutant, tsJT663, defective in preparation for passing restriction point. Sasaki T,Kameoka Y,Kamase S,Ide T Exp Cell Res. 1994 Sep;214(1):209-14
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.