JCRB0153.1 tsJT663

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0153.1 Cell Name   tsJT663
Profile   Rat embryo cell line with ts mutation for cell cycle progression from G1 to S phase. Other Name   tsJT663(3Y1)
Animal   rat Strain   Fischer 344
Genus   Rattus Species   norvegicus
Sex   F Age   fetus
Identity    Tissue for Primary Cancer   embryo
Case history   normal Metastasis   
Tissue Metastasized    Genetics   A ts mutant for cell cycle progression from G1 to S phase.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   MNNG-mutagenized 3Y1 cell derivatives with ts mutation grows at 34 C but the growth is arrested at 40 C.
Classify   mutant Established by   Nihomiya-Tsuji,J. and Ide.T.
Registered by   Kyoizumi,S. Regulation for Distribution   free
Comment    Year   2000
Medium   Dulbecco's modified Eagle's medium with 0.1 % glucose and 10% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.125% trypsin and 0.01% EDTA. Gentle piptting may be reauired to detach cells from dishes. Subculture once a week at split ratio=1/8 at 34 C.
Cell Number on Passage   5-8 x 10^5 cells/60mm dish Race   
CO2 Conc.   5 % Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_L711
Reference
Pubmed id:8082723Isolation of a mammalian cell cycle ts mutant, tsJT663, defective in preparation for passing restriction point.
Sasaki T,Kameoka Y,Kamase S,Ide T
Exp Cell Res. 1994 Sep;214(1):209-14

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0153.1 Cell Name   tsJT663
LOT No.   05022001 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum (Intergen RB52405). Temperature   34 C (ts)
Cell Density at Seeding   7.0 x 10^3 cells/sq.cm. Methods for Passages   Cells harvesed after treatment with 0.125% trypsin and 0.01% EDTA. Subculture once a week and medium change twice a week.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.2 x 10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   P8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Isozyme LDH, NP, G6PD. Rat.
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Culture medium containing 5% DMSO. CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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