JCRB0104.1 KU812E
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0104.1 | Cell Name | KU812E |
|---|---|---|---|
| Profile | leukemia, chronic myeloid | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 38 |
| Identity | available | Tissue for Primary Cancer | hemo-lymphocytic |
| Case history | chronic myeloid leukemia (CML) | Metastasis | |
| Tissue Metastasized | Genetics | Ph1 chr., histamin(+),t(9;22)(q34;q11),i(17q) etc. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | Dt=20-30hr, clonal isolation of KU812 |
| Classify | tumor | Established by | Kishi,K. |
| Registered by | Kishi,K. | Regulation for Distribution | |
| Comment | This cell line was deposited to ATCC, ECACC and DSM at 1990. | Year | 1987 |
| Medium | RPMI1640 medium with 10% fetal calf serum | Methods for Passages | Dilution. |
| Cell Number on Passage | 10^5 cells/ml | Race | Japanese |
| CO2 Conc. | 5 % | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:2540861 | KU 812: a pluripotent human cell line with spontaneous erythroid terminal maturation. Nakazawa M,Mitjavila MT,Debili N,Casadevall N,Mayeux P,Rouyer-Fessard P,Dubart A,Roméo PH,Beuzard Y,Kishi K Blood. 1989 May 15;73(7):2003-13 |
| Pubmed id:2436686 | A monoclonal antibody reacting with human basophils. Bodger MP,Mounsey GL,Nelson J,Fitzgerald PH Blood. 1987 May;69(5):1414-8 |
| Pubmed id:3695564 | Induction of differentiation of the human leukemia cell line, KU812. Fischkoff SA,Kishi K,Benjamin WR,Rossi RM,Hoessly MC,Hoxie JA,Seals C,Anderson L,Ishizaka T,Hakimi J Leuk Res. 1987;11(12):1105-13 |
| Pubmed id:3858609 | A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Kishi K Leuk Res. 1985;9(3):381-90 |
| Images |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0104.1 | Cell Name | KU812E |
|---|---|---|---|
| LOT No. | 040290 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 10^5 cells/ml | Methods for Passages | Dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.8x10^6 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P9 | PDL | |
| Sterility: MYCOPLASMA | NT | Sterility: BACTERIA | NT |
| Sterility: FUNGI | NT | Isozyme Analysis | NT |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0104.1 | Cell Name | KU812E |
|---|---|---|---|
| LOT No. | 082087 | Lot Specification | distribution |
| Medium | RPMI1640 + FCS 10% | Temperature | 37 |
| Cell Density at Seeding | 10^5 cells/ml | Methods for Passages | Dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 7x10^5 |
| Viability at cell freezing (%) | NT | Antibiotics Used | free |
| Passage Number | P4* | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | G6PD,LD,NP |
| Chromosome Mode | Chromosome Information | NT | |
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Exoteric Gene | ||
| Medium for Freezing | CO2 Conc. | ||
| Viability immediately after thawing (%) | Additional information |
| Cell No. | JCRB0104.1 | Cell Name | KU812E |
|---|---|---|---|
| LOT No. | 12102015 | Lot Specification | distribution |
| Medium | RPMI-1640 medium with 10% fetal bovine serum (FBS; GIBCO cat. # 10091) | Temperature | 37 C |
| Cell Density at Seeding | approx. 2 x 10^5 cells/mL | Methods for Passages | Simple dilution (suspension culture) |
| Doubling Time | approx 2 days | Cell Number in Vial (cells/1ml) | 2.7 x 10^6 |
| Viability at cell freezing (%) | 87 | Antibiotics Used | free |
| Passage Number | Unknown (6 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | No | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - RPMI1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87 | Additional information |