-Detailed Information [JCRB0098]-

JCRB0098 KURAMOCHI

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0098	Cell Name	KURAMOCHI
Profile	undifferentiated carcinoma, ascites	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	F	Age	unk
Identity	available	Tissue for Primary Cancer	ovary
Case history	ovarian cancer, undifferentiated carcinoma	Metastasis
Tissue Metastasized		Genetics	hyperdiploid
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	Dt=24-27hr
Classify	tumor	Established by	Kaneda
Registered by	Minato,K.	Regulation for Distribution
Comment	Mycoplasma contamination was detected when submitted to NIHS and eliminated at 1986.	Year	1986
Medium	RPMI1640 medium with 10% fetal calf serum	Methods for Passages	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage	split ratio=1/10	Race	Japanese
CO2 Conc.	5 %	Tissue Sampling	ascites
Tissue Type

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_1345
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	909975

Reference
Pubmed id:7069245	[Quantitative analysis on in vitro drug sensitivity of cultured human ovarian cancer cell lines (author's transl)]. Motoyama T Nihon Sanka Fujinka Gakkai Zasshi. 1982 Mar;34(3):308-14
Pubmed id:6268719	[Biological characterization including sensitivity to mitomycin C of cultured human ovarian cancers (author's transl)]. Motoyama T Nihon Sanka Fujinka Gakkai Zasshi. 1981 Aug;33(8):1197-204

Images

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

10242008
090298
082289
12132013
06302015
06152016
09042020
10262022
12052023
12062023

Cell No.	JCRB0098	Cell Name
LOT No.	10242008	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (GIBCO Cat. # 10099, lot 484375)	Temperature	37 C
Cell Density at Seeding	1.5 x 10^5 cells/mL	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	60 hrs	Cell Number in Vial (cells/1ml)	6.2 x 10^6
Viability at cell freezing (%)	95.8	Antibiotics Used	free
Passage Number	Unknown (17 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	090298	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal calf serum (Mitsubishi PVF01).	Temperature	37 C
Cell Density at Seeding	1.8 x 10^4 cells/sq.cm.	Methods for Passages	Cells harvested after treatment with 0.25% trypsin and 0.02% EDTA for 5 minutes at room temperature.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.2 x 10^6
Viability at cell freezing (%)	96.1	Antibiotics Used	free
Passage Number	P16*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD(typeB), NP and LDH examined. human, non-HeLa.
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:9,12 D7S820:10,11 D16S539:10 VWA:16,19 TH01:9 AM:X TPOX:8,12 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	Culture medium with 5% DMSO.	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Images

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	082289	Lot Specification	distribution
Medium	RPMI1640 + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/10	Methods for Passages	0.02 % EDTA and 0.25 % tripsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.0x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P16*	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	12132013	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37C
Cell Density at Seeding	0.6 - 1.2 x 10^5 cells/mL	Methods for Passages	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Doubling Time		Cell Number in Vial (cells/1ml)	2.1 x 10^6 cells/1mL
Viability at cell freezing (%)		Antibiotics Used	free
Passage Number	Unknown (16 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS- RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	94.5	Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	06302015	Lot Specification	distribution
Medium	RPMI1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10091)	Temperature	37 C
Cell Density at Seeding	0.3 - 1.2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 43 hrs.	Cell Number in Vial (cells/1ml)	3.0 x 10^6
Viability at cell freezing (%)	93.4	Antibiotics Used	free
Passage Number	P17	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:9,12 D7S820:10,11 D16S539:10 VWA:16,19 TH01:9 AM:X TPOX:8,12 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	93.4	Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	06152016	Lot Specification	distribution
Medium	RPMI-1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.5 - 1.2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 40 hrs.	Cell Number in Vial (cells/1ml)	1.5 x 10^6
Viability at cell freezing (%)	81.9	Antibiotics Used	free
Passage Number	Unknown (16 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI-1640	CO2 Conc.	5%
Viability immediately after thawing (%)	81.9	Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	09042020	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	0.6 - 2.7 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 2 days	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	Unknlown (17 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI 1640	CO2 Conc.	5%
Viability immediately after thawing (%)	91	Additional information

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	10262022	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437)	Temperature	37 C
Cell Density at Seeding	6.2 - 9.9 x 10^4 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 42 hrs.	Cell Number in Vial (cells/1ml)	1.7 x 10^6
Viability at cell freezing (%)	98	Antibiotics Used	free
Passage Number	Unknown (16 at bank)	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12 D13S317:9,12 D7S820:10,11 D16S539:10 VWA:16,19 TH01:9 AM:X TPOX:8,12 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - RPMI1640	CO2 Conc.	5%
Viability immediately after thawing (%)	88	Additional information

Images

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	12052023	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1.5-4.0x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.2x10^6
Viability at cell freezing (%)	96.7	Antibiotics Used	free
Passage Number	p17*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:9,12 D7S820:10,11 D16S539:10 VWA:16,19 TH01:9 AM:X TPOX:8,12 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	92.0	Additional information

Images

Cell No.	JCRB0098	Cell Name	KURAMOCHI
LOT No.	12062023	Lot Specification	distribution
Medium	RPMI 1640 medium with 10% fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K)	Temperature	37 C
Cell Density at Seeding	1.5-4.0x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.6x10^5
Viability at cell freezing (%)	98.2	Antibiotics Used	free
Passage Number	p17*	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12 D13S317:9,12 D7S820:10,11 D16S539:10 VWA:16,19 TH01:9 AM:X TPOX:8,12 CSF1PO:11,12
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER (LYMPHOTEC Inc.,CS-02-001, NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	94.2	Additional information