-Detailed Information [JCRB0070]-

JCRB0070 MIA PaCa-2

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.	JCRB0070	Cell Name	MIA PaCa-2
Profile	pancreatic cancer	Other Name
Animal	human	Strain
Genus	Homo	Species	sapiens
Sex	M	Age	65
Identity	available	Tissue for Primary Cancer	pancreas
Case history	pancreatic cancer	Metastasis
Tissue Metastasized		Genetics
Life Span	infinite	Crisis PDL
Morphology	epithelial-like	Character	sensitive to asparginase. Dt=40hr, colony-formaing efficiency in soft agar = 19%
Classify	tumor	Established by	Yunis,A. et al.
Registered by	Akiyama,M.	Regulation for Distribution	free
Comment		Year	1985
Medium	Eagle's minimal essential medium with non-essential a.a. and 10% fetal calf serum(heat inactivated)	Methods for Passages	Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage	split ratio=1/3 - 1/4	Race
CO2 Conc.	5 %	Tissue Sampling	pancreas
Tissue Type	pancreatic cancer

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus	tested				Detected RNA Virus	tested
	CMV	-	parvoB19	-		HCV	-	HTLV-1	-
	EBV	-	HBV	-		HIV-1	-	HTLV-2	-
	HHV6	-	HTLV-1	-		HIV-2	-	HAV	-
	HHV7	-	HTLV-2	-		-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.)
	BKV	-	HIV-1	-
	JCV	-	HIV-2	-
	ADV	-	HPV18	-
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized.	CVCL_0428
COSMIC CELL LINE COSMIC (Catalogue Of Somatic Mutations In Cancer) is an online database of somatically acquired mutations found in human cancer. Especially in the Cell Lines Project, mutation profiles of more than 1000 cell lines are being developed for use in cancer research. For the cell lines registered in the JCRB cell bank, you can obtain information from the right link.	724870

Reference
Pubmed id:15590	Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Wu M,Arimura GK,Yunis AA Biochemistry. 1977 May 3;16(9):1908-13
Pubmed id:832918	Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Yunis AA,Arimura GK,Russin DJ Int J Cancer. 1977 Jan;19(1):128-35

Images

Movies

LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.

11162009
04232001
092493
050891
082889
033187
01272017
11132020
07072022

Cell No.	JCRB0070	Cell Name
LOT No.	11162009	Lot Specification	distribution
Medium	Eagle's minimum essential medium with non essential amino acids (0.1 mM) and 10% fetal bovine serum (GIBCO Cat. # 10099)	Temperature	37 C
Cell Density at Seeding	approx. 1 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA
Doubling Time	20-23 hrs	Cell Number in Vial (cells/1ml)	2.9 x 10^6
Viability at cell freezing (%)	98.6	Antibiotics Used	free
Passage Number	P154	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B). MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name
LOT No.	04232001	Lot Specification	distribution
Medium	Eagle's minimal essential medium with non-essential amino acids and 10% fetal calf serum	Temperature	37 C
Cell Density at Seeding	2-5 x 10^5 cells/ml	Methods for Passages	0.25% trypsin and 0.02% EDTA in Ca, Mg-free PBS
Doubling Time		Cell Number in Vial (cells/1ml)	3.5 x 10^6
Viability at cell freezing (%)	77	Antibiotics Used	free
Passage Number	P155	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	Confirmed as human by NP, G6PD (type B), MD.
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	092493	Lot Specification	distribution
Medium	Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum	Temperature	37 C
Cell Density at Seeding	1x10^6 cells/10 cm plate	Methods for Passages	Cells are treated with 0.05% trypsin and 0.02% EDTA.
Doubling Time		Cell Number in Vial (cells/1ml)	2.14x10^6
Viability at cell freezing (%)	79.0	Antibiotics Used	free
Passage Number	P151	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	050891	Lot Specification	distribution
Medium	MEM with NEAA + FCS 10%	Temperature	37 C
Cell Density at Seeding	dilution 1/3 - 1/4	Methods for Passages	0.02% EDTA and 0.25% Trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.3x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P153	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	NT
Sterility: FUNGI	NT	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	082889	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/3 - 1/4	Methods for Passages	0.02% EDTA and 0.25% Trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3.4x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P152	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	033187	Lot Specification	distribution
Medium	MEM w/ non essential amino acids + FCS 10%	Temperature	37
Cell Density at Seeding	dilution 1/3 - 1/4	Methods for Passages	0.02% EDTA and 0.25% Trypsin.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	3x10^6
Viability at cell freezing (%)	NT	Antibiotics Used	free
Passage Number	P151	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	G6PD,LD,NP
Chromosome Mode		Chromosome Information	NT
Surface Antigen		DNA Profile (STR)
Adhesion		Exoteric Gene
Medium for Freezing		CO2 Conc.
Viability immediately after thawing (%)		Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	01272017	Lot Specification	distribution
Medium	Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Sigma Cat. # 172012)	Temperature	37 C
Cell Density at Seeding	1.4 - 2.1 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 22 hrs	Cell Number in Vial (cells/1ml)	3.4 x 10^6
Viability at cell freezing (%)	95.8	Antibiotics Used	free
Passage Number	P154	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - culture medium	CO2 Conc.	5%
Viability immediately after thawing (%)	NT	Additional information

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	11132020	Lot Specification	distribution
Medium	MEM(GIBCO cat.No.11095-080) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820)	Temperature	37 C
Cell Density at Seeding	1.91-4.32x10^4 cells/sq.cm	Methods for Passages	Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time	NT	Cell Number in Vial (cells/1ml)	2.42x10^6
Viability at cell freezing (%)	96	Antibiotics Used	free
Passage Number	p154	PDL	NT
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis	NT
Chromosome Mode	NT	Chromosome Information	NT
Surface Antigen	NT	DNA Profile (STR)	D5S818:12,13 D13S317:12,13 D7S820:12,13 D16S539:10,13 VWA:15 TH01:9,10 AM:X TPOX:9 CSF1PO:10
Adhesion	Yes	Exoteric Gene	NT
Medium for Freezing	BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)	CO2 Conc.	5%
Viability immediately after thawing (%)	85.9	Additional information

Images

Cell No.	JCRB0070	Cell Name	MIA PaCa-2
LOT No.	07072022	Lot Specification	distribution
Medium	Eagle's minimum essential medium with non-essential amino acids and 10% fetal bovine serum (FBS; Biowest Cat. # S1820-500)	Temperature	37 C
Cell Density at Seeding	approx. 2 x 10^5 cells/mL	Methods for Passages	Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time	approx. 35 hrs.	Cell Number in Vial (cells/1ml)	1.3 x 10^6
Viability at cell freezing (%)	98.6	Antibiotics Used	free
Passage Number	P154	PDL
Sterility: MYCOPLASMA	-	Sterility: BACTERIA	-
Sterility: FUNGI	-	Isozyme Analysis
Chromosome Mode		Chromosome Information
Surface Antigen		DNA Profile (STR)	D5S818:12,13 D13S317:12,13 D7S820:12,13 D16S539:10,13 VWA:15 TH01:9,10 AM:X TPOX:9 CSF1PO:10
Adhesion	Yes	Exoteric Gene
Medium for Freezing	10% DMSO, 20% FBS - EMEM with NEAA	CO2 Conc.	5%
Viability immediately after thawing (%)	96.9	Additional information