IFO50496 PC-12TG Cell information
Cell type:general cells
(View Pricing Information )
JCRB No.
IFO50496
Cell Name
PC-12TG
Profile
HAT-sensitive, 6-thioguanine-resistant.
Other Name
Animal
rat
Strain
Genus
Rattus
Species
norvegicus
Sex
Age
Identity
Tissue for Primary Cancer
neural
Case history
pheochromocytoma
Metastasis
Tissue Metastasized
Genetics
HPRT-deficient
Life Span
infinite
Crisis PDL
Morphology
epithelial-polygonal
Character
pheochromocytoma, HPRT-deficient PC-12 cell line
Classify
mutant
Established by
Satoh, M. and Takeuchi, M.
Registered by
Satoh,M.
Regulation for Distribution
Comment
Year
1998
Medium
Dulbecco's modified Eagle's medium with 10% fetal bovine serum. For selection, add 5 ug/ml 6-thioguanine.
Methods for Passages
trypsin-EDTA
Cell Number on Passage
Race
CO2 Conc.
5 %
Tissue Sampling
adrenal medulla
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.
IFO50496
Cell Name
PC-12TG
LOT No.
1090
Lot Specification
distribution
Medium
D.MEM, 90% ; FBS, 10%
Temperature
37 C
Cell Density at Seeding
Methods for Passages
trypsin-EDTA
Doubling Time
38 hr
Cell Number in Vial (cells/1ml)
2 x 10^6
Viability at cell freezing (%)
94
Antibiotics Used
free
Passage Number
P12 after subcloning
PDL
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
Chromosome Mode
Chromosome Information
Surface Antigen
DNA Profile (STR)
Adhesion
Exoteric Gene
Medium for Freezing
10% DMSO, 20% serum (final conc.) - culture medium
CO2 Conc.
Viability immediately after thawing (%)
Additional information