IFO50283 IMR-32

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50283 Cell Name   IMR-32
Profile   Human nueroblastoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   13-month-old
Identity   available Tissue for Primary Cancer   neural
Case history   neuroblastoma arizing at abdomen Metastasis   
Tissue Metastasized    Genetics   N-myc amplification was reported.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   transplantable to nude mice
Classify   tumor Established by   Nichols, W. W. et al.
Registered by   Kaisho,Y. Regulation for Distribution   
Comment    Year   1990
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat-inactivated FBS Methods for Passages   trypsin-EDTA
Cell Number on Passage    Race   Caucasian
CO2 Conc.   5 % Tissue Sampling   
Tissue Type   sympatho-adrenal cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0346
Reference
Pubmed id:2121721Monoclonal antibodies to disialogangliosides: characterization of antibody-mediated cytotoxicity against human melanoma and neuroblastoma cells in vitro.
Kawashima I,Tada N,Fujimori T,Tai T
J Biochem. 1990 Jul;108(1):109-15
Pubmed id:841332Enzyme polymorphisms as genetic signatures in human cell cultures.
O'Brien SU,Kleiner G,Olson R,Shannon JE
Science. 1977 Mar 25;195(4284):1345-8
Pubmed id:5459762Definition of a continuous human cell line derived from neuroblastoma.
Tumilowicz JJ,Nichols WW,Cholon JJ,Greene AE
Cancer Res. 1970 Aug;30(8):2110-8

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50283 Cell Name   
LOT No.   12022005 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids and 10% heat-inactivated fetal bovine serum (FBS lot; GIBCO 4218333S) Temperature   37 C
Cell Density at Seeding   0.6 - 1.4 x 10^5 cells/ml Methods for Passages   0.25% trypsin and 0.02% EDTA. (Alternatively, cells can be detached with Ca, Mg-free PBS treatment.)
Doubling Time   approx. 39 hrs Cell Number in Vial (cells/1ml)   2.8 x 10^6
Viability at cell freezing (%)   97.4 Antibiotics Used   free
Passage Number   P60 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as human by NP, G6PD (type B), LD.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:11,12
D13S317:9
D7S820:9,10
D16S539:8
VWA:15
TH01:7,9.3
AM:X,Y
TPOX:11
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - culture medium CO2 Conc.   5%
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50283 Cell Name   IMR-32
LOT No.   1059 Lot Specification   distribution
Medium   E.MEM with NEAA, 90% ; heat-inactivated FBS, 10% Temperature   37 C
Cell Density at Seeding    Methods for Passages   trypsin-EDTA
Doubling Time    Cell Number in Vial (cells/1ml)   2 x 10^6
Viability at cell freezing (%)   89 Antibiotics Used   free
Passage Number   P57 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD(type B), PepB
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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