IFO50278 PC-12

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   IFO50278 Cell Name   PC-12
Profile   pheochromocytoma, neuronal differentiation by NGF, grow in suspension Other Name   PC12
Animal   rat Strain   
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   neural
Case history   pheochromocytoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round Character   pheochromocytoma, neuronal differentiation by NGF
Classify   tumor Established by   Greene, L. & Tischler, A.
Registered by   Nakahama,K. Regulation for Distribution   
Comment    Year   1990
Medium   RPMI1640 medium with 10% heat-inactivated horse serum and 5% fetal bovine serum Methods for Passages   pipetting
Cell Number on Passage    Race   
CO2 Conc.   5 % Tissue Sampling   adrenal medulla
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_0481
Reference
Pubmed id:3839317Molecular cloning of a gene sequence regulated by nerve growth factor.
Levi A,Eldridge JD,Paterson BM
Science. 1985 Jul 26;229(4711):393-5
Pubmed id:1065897Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.
Greene LA,Tischler AS
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2424-8
Pubmed id:15437183Production of malignancy in vitro. XI. Further results from reinjection of in vitro cell strains into strain C3H mice.
EARLE WR,SHELTON E,SCHILLING EL
J Natl Cancer Inst. 1950 Apr;10(5):1105-13

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   IFO50278 Cell Name   
LOT No.   05212001 Lot Specification   distribution
Medium   RPMI1640 medium with 10% heat-inactivated horse serum and 5% fetal bovine seurm Temperature   37 C
Cell Density at Seeding   0.5-2 x 10^5 cells/ml Methods for Passages   Pipetting (suspension culture)
Doubling Time    Cell Number in Vial (cells/1ml)   2.7 x 10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   P7* PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   Confirmed as rat by NP, G6PD, MD, AST.
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing    CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50278 Cell Name   PC-12
LOT No.   1084 Lot Specification   distribution
Medium   RPMI 1640, 85% ; heat-inactivated horse serum, 10% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   pipetting
Doubling Time    Cell Number in Vial (cells/1ml)   8 x 10^5
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (6 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NP, G6PD, AST
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
Cell No.   IFO50278 Cell Name   
LOT No.   507 Lot Specification   distribution
Medium   RPMI 1640, 85% ; heat-inactivated horse serum, 10% ; FBS, 5% Temperature   37 C
Cell Density at Seeding    Methods for Passages   pipetting
Doubling Time    Cell Number in Vial (cells/1ml)   1 x 10^6
Viability at cell freezing (%)   93 Antibiotics Used   free
Passage Number   Unknown (7 at IFO) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   G6PD, MD, MPI
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion    Exoteric Gene   
Medium for Freezing   10% DMSO, 20% serum (final conc.) - culture medium CO2 Conc.   
Viability immediately after thawing (%)    Additional information   
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