AyuK12A07 Saps3-K1

Cell information

Cell type：mousehomozygous mutant ES cells (View Pricing Information)

JCRB No.   AyuK12A07 Cell Name   Saps3-K1
Profile   Homozygous mutant mouse embryonic stem cell. Deficient gene: Saps3 Other Name   K12A07
Animal   mouse Strain   (C57BL/6 x 129S4/SvJae)F1
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   embryo
Case history    Metastasis   
Tissue Metastasized    Genetics   Saps3, SAPS domain family, member 3
Life Span   infinite Crisis PDL   
Morphology   ES-like Character   pCMT-SAhygpA-NP21
Classify   Homozygous mutant Established by   Horie, K.
Registered by   Horie, K. Regulation for Distribution   
Comment    Year   
Medium   Knockout DMEM medium with 20% FBS, NEAA, sodium pyruvate, 0.1mM 2-mercaptoethanol, 1000U/mL LIF on mitomycin C-treated MEF feeder cells. Methods for Passages   0.125% trypsin+0.01% EDTA, 0.1% gelatin coated dish
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_E5Y9



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   AyuK12A07 Cell Name   Saps3-K1
LOT No.   10302015 Lot Specification   distribution
Medium   Knockout DMEM with 20% fetal bovine serum(BioWest Lot.S09336S1820,non HI),2mM glutamine,NEAA,sodium pyruvate,0.1mM 2-mercaptoethanol and 1000U/ml LIF on mitomycin C-treated MEF feeder cells. Temperature   37 C
Cell Density at Seeding   0.5-5.0x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 0.01% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.95x10^6
Viability at cell freezing (%)   92.1 Antibiotics Used   penicillin:100 units/ml , streptomycin:100 ug/ml
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Bambanker(Lymphotec Inc.) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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