JCRB4189 SP-53
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4189 | Cell Name | SP-53 |
|---|---|---|---|
| Profile | Mantle lymphoma cell line (EBV negative), same patient origin as SP-50B | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 58-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | t(11;14)(q13;q32) chromosomal abnormality | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | |
| Classify | tumor | Established by | Masanori DAIBATA |
| Registered by | Masanori DAIBATA | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI 1640 medium supplemented with 10% FBS. | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood |
| Tissue Type | mantle cell lymphoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:2548699 | The establishment of Epstein-Barr virus nuclear antigen-positive (SP-50B) and Epstein-Barr virus nuclear antigen-negative (SP-53) cell lines with t(11;14)(q13;q32) chromosome abnormality from an intermediate lymphocytic lymphoma. Daibata M,Kubonishi I,Eguchi T,Yano S,Ohtsuki Y,Miyoshi I Cancer. 1989 Sep 15;64(6):1248-53 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4189 | Cell Name | SP-53 |
|---|---|---|---|
| LOT No. | 04062026 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-2.6x10^5 cells/mL | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.5x10^6 |
| Viability at cell freezing (%) | 84 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:9,10 D7S820:7,12 D16S539:9,11 VWA:14,16 TH01:7,9 AM:X TPOX:8,9 CSF1PO:12,13 |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | Cell Banker 1 (Nihon Zenyaku Kogyo Co.Ltd Catalog No. CB011) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 80 | Additional information |
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