JCRB4186 UMK-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4186 | Cell Name | UMK-1 |
|---|---|---|---|
| Profile | Bladder carcinoma cell line | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 61-year-old |
| Identity | available | Tissue for Primary Cancer | bladder |
| Case history | Bladder carcinoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | tumor | Established by | |
| Registered by | Makiko KAWAGUCHI | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640:Ham's F12=1:1 medium with 10% FBS | Methods for Passages | 0.25% Trypsin + 0.02% EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | bladder |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7610502 | Establishment of a new human urinary bladder mixed carcinoma cell line UMK-1 and its cell-density-dependent secretion of gelatinases and tissue inhibitor of metalloproteinase-1. Muraoka K,Nabeshima K,Kataoka H,Kishi J,Koono M Urol Int. 1995;54(4):184-90 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4186 | Cell Name | UMK-1 |
|---|---|---|---|
| LOT No. | 02252026 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12 medium (GIBCO Cat#11765-054) (1/1) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 1.14-2.18x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.30x10^6 |
| Viability at cell freezing (%) | 99.21 | Antibiotics Used | free |
| Passage Number | p23 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11 D13S317:11 D7S820:10 D16S539:12 VWA:14,18 TH01:9 AM:X,Y TPOX:8,9 CSF1PO:10 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.08 | Additional information |
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