JCRB4185 AIA3
Cell information
Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB4185 | Cell Name | AIA3 |
|---|---|---|---|
| Profile | An immortalized adipose stromal cell line transfected with TERT, CDK4R24C (mutant CDK4: an INK4a-resistant form of CDK4), and CCND1 genes. Differentiation into mature adipocytes, osteoblasts, and chondrocytes is possible. Japanese origin. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 63-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | Gene transfer of TERT gene, CDK4R24C (mutant CDK4: an INK4a-resistant form of CDK4) gene and CCND1 gene using lentiviral vectors | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | transformed | Established by | Hirohiko AKAMATSU |
| Registered by | Hirohiko AKAMATSU | Regulation for Distribution | |
| Comment | Year | ||
| Medium | DMEM and α minimum essential medium (α MEM, Invitrogen) with a 1:1 ratio, supplemented with 25mM Hepes,3% GlutaMax ,1% FBS, 1× ITS-X (Invitrogen), 10 ng/mL basic fibroblast growth factor (bFGF) and 0.4 μg/mL hydrocortisone. | Methods for Passages | 0.25% Trypsin-EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:34602549 | Establishment of Three Types of Immortalized Human Skin Stem Cell Lines Derived from the Single Donor. Inoue Y,Hasegawa S,Hasebe Y,Kawagishi-Hotta M,Okuno R,Yamada T,Adachi H,Miyachi K,Ishii Y,Sugiura K,Akamatsu H Biol Pharm Bull. 2021;44(10):1403-1412 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4185 | Cell Name | AIA3 |
|---|---|---|---|
| LOT No. | 02242026 | Lot Specification | distribution |
| Medium | DMEM and MEM alpha with a 1:1 ratio, supplemented with 25mM Hepes,3% GlutaMax ,1% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K), 1× ITS-X (Invitrogen),10 ng/mL basic fibroblast growth factor (bFGF) and 0.4 μg/mL hydrocortisone. | Temperature | 37 C |
| Cell Density at Seeding | 2-5x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.03x10^6 |
| Viability at cell freezing (%) | 95.5 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:11,12 D7S820:11,12 D16S539:9,11 VWA:18,19 TH01:9 AM:X,Y TPOX:11 CSF1PO:12,13 |
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | BAMBANKER (NIPPON Genetics Co., LTD, Cat.No.CS-02-001） | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 93.0 | Additional information |