JCRB4118 MRT-1
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4118 | Cell Name | MRT-1 |
|---|---|---|---|
| Profile | Cell lines derived from lymph node metastases of renal cell carcinoma | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 47-year-old |
| Identity | available | Tissue for Primary Cancer | kidney |
| Case history | renal cell carcinoma | Metastasis | Y |
| Tissue Metastasized | lymph node | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | tumor | Established by | Keisuke MURAOKA |
| Registered by | Makiko KAWAGUCHI | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640:Ham's F12=1:1 medium with 10% FBS | Methods for Passages | 0.25% Trypsin + 0.02% EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | lymph node |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:7669747 | Establishment and characterization of a human renal carcinoma cell line MRT-1, with special reference to the production of serine proteinase inhibitors. Muraoka K,Kataoka H,Nabeshima K,Seguchi K,Moriyama T,Osada Y,Koono M Hum Cell. 1995 Mar;8(1):11-8 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4118 | Cell Name | MRT-1 |
|---|---|---|---|
| LOT No. | 01272026 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO) and Ham's F12medium (GIBCO Cat#11765-054) (1/1) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 0.58-1.89x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.00x10^6 |
| Viability at cell freezing (%) | 100.00 | Antibiotics Used | free |
| Passage Number | p41 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:13 D13S317:11,12 D7S820:11,12 D16S539:9,13 VWA:17,18 TH01:6,9 AM:X,Y TPOX:8,11 CSF1PO:10,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 96.48 | Additional information |