JCRB4114 HPL-EsC-1-M
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4114 | Cell Name | HPL-EsC-1-M |
|---|---|---|---|
| Profile | Cell line derived from patients with esophageal cancer, poorly differentiated squamous cell carcinoma | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | esophagus |
| Case history | esophageal cancer | Metastasis | Y |
| Tissue Metastasized | pleural effusion | Genetics | |
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | EGFR(+), Pemphigus antigen(+), Ia antigen, Epithelial-like monolayer growth, Transplantable to nude mice (Forming large lesions of differentiated squamous cell carcinoma) |
| Classify | tumor | Established by | Shigeru Morikawa |
| Registered by | Toru Nabika | Regulation for Distribution | |
| Comment | This cell line was found to be contaminated by mycoplasmas at the deposition of cell bank. The mycoplasmas were eliminated by the treatment with MC-210 by JCRB Cell Bank. The current lots for distribution are mycoplasma-free. | Year | |
| Medium | RPMI1640+10% FBS | Methods for Passages | 0.25% Trypsin - 0.02% EDTA |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | pleural effusion |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1467327 | [Development of an experimental model for spontaneous lymph node metastasis of human esophageal carcinoma in nude mice--histopathological analysis]. Shigetomi A,Kotoh T,Harada T,Morikawa S,Nakamura T Hum Cell. 1992 Sep;5(3):273-81 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4114 | Cell Name | HPL-EsC-1-M |
|---|---|---|---|
| LOT No. | 02272026 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 0.12-1.1x10^5 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.1x10^6 |
| Viability at cell freezing (%) | 98 | Antibiotics Used | free |
| Passage Number | ｐ17* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:8 D7S820:9 D16S539:9,10 VWA:16,21 TH01:7,9 AM:X TPOX:11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 58 | Additional information |
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