JCRB4108 MDCK-5102HRasCT(Picchu)

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB4108 Cell Name   MDCK-5102HRasCT(Picchu)
Profile   MDCK cells with a tyrosine kinase FRET biosensor (5102HRasCT(Picchu)) Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   tyrosine kinase FRET biosensor (5102HRasCT(Picchu)) expressing. Used vector: pPBbsr2-5102HRasCT(Picchu)
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Eriko DEGUCHI
Registered by   Michiyuki MATSUDA Regulation for Distribution   
Comment    Year   
Medium    DMEM with 10% FBS Methods for Passages   0.25% trypsin + 0.02% EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:39546398Low-affinity ligands of the epidermal growth factor receptor are long-range signal transmitters in collective cell migration of epithelial cells.
Deguchi E,Lin S,Hirayama D,Matsuda K,Tanave A,Sumiyama K,Tsukiji S,Otani T,Furuse M,Sorkin A,Matsuda M,Terai K
Cell Rep. 2024 Nov 14;43(11):114986

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB4108 Cell Name   MDCK-5102HRasCT(Picchu)
LOT No.   10172025 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% heat-inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   0.83-2.33x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   97.56 Antibiotics Used   free
Passage Number   p8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (NIPPON Genetics Co., LTD, Cat.No.CS-02-001） CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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