JCRB4084 UT-7
Cell information
Important Notice(s)Cultivation of UT-7 cells (JCRB4084) / UT-7細胞の培養について
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4084 | Cell Name | UT-7 |
|---|---|---|---|
| Profile | Human megakaryoblastic leukemia cell line with absolute dependence on interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or erythropoietin (EPO) for growth and survival. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 64-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | acute myeloid leukemia (AML M7) | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | megakaryoblast-like | Character | |
| Classify | tumor | Established by | Norio KOMATSU |
| Registered by | Norio KOMATSU | Regulation for Distribution | |
| Comment | Year | ||
| Medium | IMDM with 10% FCS and 1 ng/mL of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) | Methods for Passages | dilution |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1824823 | Establishment and characterization of a human leukemic cell line with megakaryocytic features: dependency on granulocyte-macrophage colony-stimulating factor, interleukin 3, or erythropoietin for growth and survival. Komatsu N,Nakauchi H,Miwa A,Ishihara T,Eguchi M,Moroi M,Okada M,Sato Y,Wada H,Yawata Y Cancer Res. 1991 Jan 1;51(1):341-8 |
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4084 | Cell Name | UT-7 |
|---|---|---|---|
| LOT No. | 07142025 | Lot Specification | distribution |
| Medium | IMDM (GIBCO) with 10% heat inactivated fetal bovine serum (Nichirei Cat. # 174112, Lot 20M00K) and 1 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor. | Temperature | 37 C |
| Cell Density at Seeding | 1.37-2.59x10^5 cells/mL | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.00x10^6 |
| Viability at cell freezing (%) | 95.83 | Antibiotics Used | free |
| Passage Number | p9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:8 D7S820:8 D16S539:10,12 VWA:14,18 TH01:6,9 AM:X,Y TPOX:10 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 72.54 | Additional information | culture the cell so that the cell concentration dose not exeed 5x10^5 cells/5mL |