JCRB4055 MEF

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB4055 Cell Name   MEF
Profile   Mouse embryonic fibroblasts isolated from B6N albino mouse embryos are immortalized by expressing SV40 LargeT with a retroviral vector (pMSCV). This cell line was used as the contol of Blvra–/– MEF, but not validated as the feeder cells for ES and iPS cells. Other Name   
Animal   mouse Strain   C57BL/6NCrl
Genus    Species   
Sex    Age   embryo
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   puromycin-resistant
Classify   transformed Established by   Kenju KOBACHI
Registered by   Michiyuki MATSUDA Regulation for Distribution   
Comment    Year   
Medium   DMEM (high-glucose) with 10% FBS Methods for Passages    Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Reference
Pubmed id:32581154Biliverdin Reductase-A Deficiency Brighten and Sensitize Biliverdin-binding Chromoproteins.
Kobachi K,Kuno S,Sato S,Sumiyama K,Matsuda M,Terai K
Cell Struct Funct. 2020 Aug 21;45(2):131-141

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LOT Information

Cell No.   JCRB4055 Cell Name   MEF
LOT No.   12042024 Lot Specification   distribution
Medium   DMEM (4.5g/L D-Glucose, GIBCO Cat#11995-065) with 10% heat inactivated fetal bovine serum (SIGMA, Cat# 172012, Lot#12J396) Temperature   37 C
Cell Density at Seeding   6.41-9.47x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.24x10^6
Viability at cell freezing (%)   87.30 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   97.38 Additional information   
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