JCRB4004 KE-43C4
Cell information
Important Notice(s)JCRB4004 KE-43C4 notes for cultivation / 培養上の注意
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB4004 | Cell Name | KE-43C4 |
|---|---|---|---|
| Profile | Well-differentiated adenocarcinoma obtained by cloning KE-43 | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 54-year-old |
| Identity | available | Tissue for Primary Cancer | colon |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | epithelial-like | Character | DT=63.6 hrs |
| Classify | tumor | Established by | Kazuya YAMASHITA |
| Registered by | YAMASHITA Kazuya | Regulation for Distribution | |
| Comment | Year | ||
| Medium | MEM+10%FBS | Methods for Passages | 0.25%Trypsin+0.02%EDTA, passage 1:10split /1 week |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | primary colonic carcinoma |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:16206288 | Disruption of the p53-p53r2 DNA repair system in ulcerative colitis contributes to colon tumorigenesis. Yoshida T,Haga S,Numata Y,Yamashita K,Mikami T,Ogawa T,Ohkusa T,Okayasu I Int J Cancer. 2006 Mar 15;118(6):1395-403 |
| Pubmed id:15016319 | Unique characteristics of rectal carcinoma cell lines derived from invasive carcinomas in ulcerative colitis patients. Yamashita K,Yasuda S,Kuba T,Otani Y,Fujiwara M,Okayasu I Cancer Sci. 2004 Mar;95(3):211-7 |
| Pubmed id:1390419 | [Establishment and characterization of a human colonic cancer cell line expressing carbohydrate antigen 19-9 and carcinoembryonic antigen]. Atari E,Watanabe K,Yamashita K Hum Cell. 1992 Jun;5(2):174-9 |
| Pubmed id:none | 細胞株を用いた免疫組織化学染色マルチプルコントロール試料の作製と標準化の基礎的検討. 山下和也, 他． 神奈川県臨床検査技師会雑誌(2188-6628) |
| Pubmed id:none | ヒト大腸癌培養細胞株の腹腔内増殖能と癌関連遺伝子および癌関連蛋白異常に関する検討． 西山保比古、他． 日本大腸肛門病学会雑誌、50: 562- 571, 1997. |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB4004 | Cell Name | KE-43C4 |
|---|---|---|---|
| LOT No. | 03112024 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium (GIBCO:catalog No.11885-084) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396). | Temperature | 37 C |
| Cell Density at Seeding | 0.36-1.6x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (1/10 split/week) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.8x10^6 |
| Viability at cell freezing (%) | 97.90 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,12 D13S317:9 D7S820:8,10 D16S539:9,11 VWA:14,18 TH01:6 AM:X TPOX:8 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 95.5 | Additional information | We recommend to start culture with 25T flask. |
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