JCRB1980 MDCK-EKARrEV-NLS-dEREG

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1980 Cell Name   MDCK-EKARrEV-NLS-dEREG
Profile   epiregulin (EREG)-deficient MDCK cell line expressing FRET biosensor EKARrEV-NLS. Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   mutation of EREG and FRET biosensor EKARrEV-NLS expressing
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Shuhao Lin
Registered by   Michiyuki MATSUDA Regulation for Distribution   
Comment    Year   
Medium   DMEM with 10% FBS Methods for Passages   0.25% trypsin + 0.02% EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D5IZ
Reference
Pubmed id:34667080Redundant roles of EGFR ligands in the ERK activation waves during collective cell migration.
Lin S,Hirayama D,Maryu G,Matsuda K,Hino N,Deguchi E,Aoki K,Iwamoto R,Terai K,Matsuda M
Life Sci Alliance. 2022 Jan;5(1):

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1980 Cell Name   MDCK-EKARrEV-NLS-dEREG
LOT No.   10242023 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium (GIBCO cat No. 11885) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396). Temperature   37 C
Cell Density at Seeding   9.09x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.86x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion    Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   96 Additional information   
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