JCRB1972 Kit225

Cell information

Important Notice(s)

Kit225細胞の分譲について：営利機関は細胞樹立者とのMTA締結が必要です

Requirement of MTA between customers and developer for the distribution of Kit225 cells to profit-making organizations.

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1972 Cell Name   Kit225
Profile   IL-2 dependent T-cell chronic lymphocytic leukemia (T-CLL) cell line Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   62-year-old
Identity   available Tissue for Primary Cancer   
Case history   T-cell chronic lymphocytic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Toshiyuki HORI
Registered by   Toshiyuki HORI Regulation for Distribution   Profit organization should conclude MTA with the developer.
Comment    Year   
Medium   RPMI1640 medium + 10%FBS with 1 U/mL human IL-2 Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   perpheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5443
Reference
Pubmed id:3115332Establishment of an interleukin 2-dependent human T cell line from a patient with T cell chronic lymphocytic leukemia who is not infected with human T cell leukemia/lymphoma virus.
Hori T,Uchiyama T,Tsudo M,Umadome H,Ohno H,Fukuhara S,Kita K,Uchino H
Blood. 1987 Oct;70(4):1069-72

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1972 Cell Name   Kit225
LOT No.   09152023 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat #11875-093) with 10% heat inactivated fetal bovine serum (SIGMA, Cat#172012, Lot#12J396) and 1 U/ml rHu IL-2 (10 U/ml until growing well) Temperature   37 C
Cell Density at Seeding   1-2.5x10^5 cells/ml Methods for Passages   Dilution (1/3-1/9)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.68x10^6
Viability at cell freezing (%)   89.6 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,12
D13S317:9,12
D7S820:8,11
D16S539:9,12
VWA:17,19
TH01:7,9
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   78.2 Additional information   It is recomended to be 10 U/ml IL-2 until growing well.
Images
CLOSE