JCRB3079 AT4BI(SV)
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3079 | Cell Name | AT4BI(SV) |
|---|---|---|---|
| Profile | Hereditary disease with high cancer risk ataxia telangiectasia (autosomal recessive) chromosome breakage syndrome. The proliferation of this cell line can not be expected. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | skin |
| Case history | Ataxia telangiectasia (C) | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | fibroblast-like | Character | SV40 transfection |
| Classify | transformed | Established by | |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | ||
| CO2 Conc. | Tissue Sampling | skin | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB3079 | Cell Name | AT4BI(SV) |
|---|---|---|---|
| LOT No. | 07182023 | Lot Specification | distribution |
| Medium | MEM alpha (GIBCO, Cat.No.12571) with 10% fetal bovine serum (not HI) (SIGMA, Cat#172012; Lot#12J396) ! Culture in 15% FBS when thawing. | Temperature | 37 C |
| Cell Density at Seeding | 1.26-2.98x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.28x10^6 |
| Viability at cell freezing (%) | 85.2 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12,13 D13S317:10,11 D7S820:8,9 D16S539:12,13 VWA:15,17 TH01:8,9.3 AM:X,Y TPOX:8,11 CSF1PO:11,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 87.77 | Additional information | 本細胞の増殖は厳しい。 Culture in 15% FBS when thawing. |
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