JCRB1959 BT-G
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1959 | Cell Name | BT-G |
|---|---|---|---|
| Profile | bovine trophoblastic cell line | Other Name | |
| Animal | bovine | Strain | |
| Genus | Bos | Species | taurus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | normal | Established by | Hashizume K., Takahashi T. |
| Registered by | Keiichiro Kizaki | Regulation for Distribution | |
| Comment | BMP4 concentration at cell establishment: 10 ng/mL | Year | |
| Medium | DMEM/F12 medium + 10% FBS (Culture in 20% FBS when thawing), Use collagen-coated dish or flask (Cellmatrix Type I-C, Nitta Gelatin, Osaka,Japan) | Methods for Passages | Pipetting dissociation and dilution. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | blastcyst |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:21862694 | Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines. Suzuki Y,Koshi K,Imai K,Takahashi T,Kizaki K,Hashizume K Reproduction. 2011 Nov;142(5):733-43 |
| Images |
|---|
      |
| Movies |
|---|
|
LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1959 | Cell Name | BT-G |
|---|---|---|---|
| LOT No. | 07112023 | Lot Specification | distribution |
| Medium | DMEM/ F12(1/1) medium (GIBCO 11320-033) with 10% heat inactivated fetal bovine serum (Japan Bio Serum:cat#NZP 001, Lot#CNGGR-100). Use type-1 collagen-coated tissue culture flask/dish. Corning: BioCoat collagen 1 coated dish / AGC TECHNO GLASS(IWAKI): Collagen Type1 coated dish | Temperature | 37 C |
| Cell Density at Seeding | Uncountable(aggregates) | Methods for Passages | Pipetting dissociation and dilution. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | Uncountable(aggregates) |
| Viability at cell freezing (%) | Uncountable(aggregates) | Antibiotics Used | free |
| Passage Number | p99 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Y/N | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | Culture in 20% FBS when thawing. Always use BVDV-free products for serum. Start culturing one ampoule in about 2 mL of culture medium (T-25 flask). |
| Images |
|---|
|
|