JCRB1935 BT-B
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1935 | Cell Name | BT-B |
|---|---|---|---|
| Profile | bovine trophoblastic cell line | Other Name | |
| Animal | bovine | Strain | |
| Genus | Bos | Species | taurus |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | |
| Classify | normal | Established by | Hashizume K., Takahashi T. |
| Registered by | Keiichiro Kizaki | Regulation for Distribution | |
| Comment | BMP4 concentration at cell establishment: 10 ng/mL | Year | |
| Medium | DMEM/F12 medium + 10% FBS (Culture in 20% FBS when thawing), Use collagen-coated dish or flask (Cellmatrix Type I-C, Nitta Gelatin, Osaka,Japan) | Methods for Passages | Pipetting dissociation and dilution. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | blastcyst |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:21862694 | Bone morphogenetic protein 4 accelerates the establishment of bovine trophoblastic cell lines. Suzuki Y,Koshi K,Imai K,Takahashi T,Kizaki K,Hashizume K Reproduction. 2011 Nov;142(5):733-43 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1935 | Cell Name | BT-B |
|---|---|---|---|
| LOT No. | 02122023 | Lot Specification | distribution |
| Medium | DMEM/ F12(1/1) medium with 10% heat inactivated fetal bovine serum (Japan Bio Serum:Cat. NZP 001, Lot: CNGGR-100) Culture in 20% FBS when thawing Use type-I collagen coated tissue culture flask/ dish. Corning: Bio Coat collagen I coated dish/ AGC TECHNO GLASS(IWAKI): Collagen Type I coated dish | Temperature | 37 C |
| Cell Density at Seeding | Uncountable(aggregates) | Methods for Passages | Pipetting dissociation and dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | Uncountable(aggregates) |
| Viability at cell freezing (%) | Uncountable(aggregates) | Antibiotics Used | free |
| Passage Number | p100 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER (LYMPHOTEC Inc., CS-02-001, NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | Uncountable | Additional information | Culture in 20% FBS when thawing Use type-I collagen coated tissue culture flask/ dish. Corning: Bio Coat collagen I coated dish/ AGC TECHNO GLASS(IWAKI): Collagen Type I coated dish |
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