JCRB1890 CHKS-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1890 Cell Name   CHKS-1
Profile   Established from hepatocellular carcinoma in dogs. Alpha-fetoprotein producing clonal cell line. Other Name   
Animal   dog Strain   
Genus   Canis Species   familiaris
Sex   M Age   11-year-old
Identity   not done Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Alpha-fetoprotein producing clonal cell line.
Classify   tumor Established by   Shinpei KAWARAI
Registered by   Shinpei KAWARAI Regulation for Distribution   
Comment    Year   2020
Medium   IMDM+10% FBS Methods for Passages   0.1%Tripsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   liver
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_FA17
Reference
Pubmed id:16678911Establishment and characterization of primary canine hepatocellular carcinoma cell lines producing alpha-fetoprotein.
Kawarai S,Hashizaki K,Kitao S,Nagano S,Madarame H,Neo S,Ishikawa T,Furuichi M,Hisasue M,Tsuchiya R,Tsujimoto H,Yamada T
Vet Immunol Immunopathol. 2006 Sep 15;113(1-2):30-6

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1890 Cell Name   CHKS-1
LOT No.   05202022 Lot Specification   distribution
Medium   IMDM(GIBCO) with 10% heat inactivated fetal bovine serum(HANA-NESCO BIO Lot# han03041) Temperature   37 C
Cell Density at Seeding   2.3-3.6x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1%trypsin(GIBCO) and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.0x10^6
Viability at cell freezing (%)   98.80 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   95.1 Additional information   We recommend to start culture with 25T flask.
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