JCRB1878 HSC-43
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1878 | Cell Name | HSC-43 |
|---|---|---|---|
| Profile | Human signet ring cell gastric carcinoma cell line. Established from primary gastric tumor. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 56-year-old |
| Identity | not done | Tissue for Primary Cancer | stomach |
| Case history | scirrhous stomach cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | Adherent cells |
| Classify | tumor | Established by | Yanagihara Kazuyoshi |
| Registered by | Yuki Iino | Regulation for Distribution | |
| Comment | Year | 2020 | |
| Medium | RPMI1640 medium + 10% FBS | Methods for Passages | 0.05% Trypsin-0.02% EDTA |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | stomach |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:8387462 | Establishment and characterization of a human gastric scirrhous carcinoma cell line in serum-free chemically defined medium. Yanagihara K,Kamada N,Tsumuraya M,Amano F Int J Cancer. 1993 May 8;54(2):200-7 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1878 | Cell Name | HSC-43 |
|---|---|---|---|
| LOT No. | 12202021 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat#11875-093) with 20% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.0.-1.5x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.05%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/5) |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 4.6x10^6 |
| Viability at cell freezing (%) | 98.10 | Antibiotics Used | free |
| Passage Number | p18 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:10,12 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:9 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 94.7 | Additional information | This cell line is not easy to detach from culture flask.Our trypsinization method is after three-time washing with PBS,then 37 ℃ 10min plus room temperature with 0.05% trypsin-EDTA and tapping Flask.Additional room temperature incubation is not necessary. |
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| Cell No. | JCRB1878 | Cell Name | HSC-43 |
|---|---|---|---|
| LOT No. | 11102023 | Lot Specification | distribution |
| Medium | RPMI 1640 medium with 20% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 1.8 - 4.5 x 10^5 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA for 10 min. |
| Doubling Time | 74 - 93 hrs. | Cell Number in Vial (cells/1ml) | 1.3 x 10^6 |
| Viability at cell freezing (%) | 92.7 | Antibiotics Used | free |
| Passage Number | P20 | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | D5S818:12 D13S317:11,12 D7S820:10,12 D16S539:9,10 VWA:16,17 TH01:9 AM:X TPOX:9 CSF1PO:12 |
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| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 5% DMSO, 20% FBS - RPMI 1640 | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 58.6 | Additional information | Cells will grow with forming islands of cell aggregates. |