JCRB1864 HSC-39

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1864 Cell Name   HSC-39
Profile   Human signet ring cell gastric carcinoma cell line establised in vitro from ascites. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   54-year-old
Identity   not done Tissue for Primary Cancer   stomach
Case history   scirrhous stomach cancer Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   round shape Character   Addition of TGF-β induces apoptosis. HSC-40A established from same patient. Floating cells.
Classify   tumor Established by   Yanagihara Kazuyoshi
Registered by   Yuki Iino Regulation for Distribution   
Comment    Year   2020
Medium   RPMI1640 medium + 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_A385
Reference
Pubmed id:1846312Establishment and characterization of human signet ring cell gastric carcinoma cell lines with amplification of the c-myc oncogene.
Yanagihara K,Seyama T,Tsumuraya M,Kamada N,Yokoro K
Cancer Res. 1991 Jan 1;51(1):381-6

Images



LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1864 Cell Name   HSC-39
LOT No.   07292021 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum (SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.93-2.94x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.02x10^6
Viability at cell freezing (%)   92 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13,14
D13S317:9
D7S820:11,12
D16S539:9
VWA:14
TH01:7,9
AM:X
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
Images
Cell No.   JCRB1864 Cell Name   HSC-39
LOT No.   02132025 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   1.1 - 3.2 x 10^5 cells/mL Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 27 hrs. Cell Number in Vial (cells/1ml)   1.9 x 10^6
Viability at cell freezing (%)   92.0 Antibiotics Used   free
Passage Number   Unknown (7 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   86.4 Additional information   
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