JCRB1837 Neuro-2a CMV-Luc
Cell information
Important Notice(s)発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines
Information for introduced genes, etc in luciferase expressing cell lines
Cell type：luciferase-expressing cancer cells (View Pricing Information)
| JCRB No. | JCRB1837 | Cell Name | Neuro-2a CMV-Luc |
|---|---|---|---|
| Profile | Luciferase stably expressing cell line (Neuro-2a; Mouse Neuroblastoma). | Other Name | |
| Animal | mouse | Strain | A albino |
| Genus | Species | ||
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | neural | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | pLVSIN-CMV-luc transfected. Luciferase stably expressing cell line. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | neuron & amoeboid-L | Character | neuroblastoma. puromycin-resistant. |
| Classify | tumor | Established by | Murakami, T. |
| Registered by | Takashi Murakami | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Eagle's minimal essential medium with non-essential amino acids and 10% fetal bovine serum. Puromycin 5.0 ug/mL | Methods for Passages | Cells are treated with 0.02 % EDTA and 0.25% trypsin. |
| Cell Number on Passage | split ratio = 1/6 - 1/20. | Race | |
| CO2 Conc. | 5% | Tissue Sampling | region of spinal cord |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1837 | Cell Name | Neuro-2a CMV-Luc |
|---|---|---|---|
| LOT No. | 12152020 | Lot Specification | distribution |
| Medium | MEM(GIBCO cat.No.11095-080) with non essential amino acid (GIBCO cat.No.11140-050) and 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) | Temperature | 37 C |
| Cell Density at Seeding | 1.16-1.64x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.77x10^6 |
| Viability at cell freezing (%) | 95.7 | Antibiotics Used | free |
| Passage Number | p4* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 53.8 | Additional information | We recommend to culture it with the medium which added Puromycin (5 ug/ml) to keep Luminescence guantity of the luciferase. |
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