JCRB3067 AP39P(SVT)
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3067 | Cell Name | AP39P(SVT) |
|---|---|---|---|
| Profile | Aplastic anemia, SV40 Ori-Transfection, lymphocyte is High SCE (sister chromatid exchange) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | |
| Identity | Tissue for Primary Cancer | skin | |
| Case history | Aplastic anemia, (lymphocyte is High SCE) | Metastasis | |
| Tissue Metastasized | Genetics | lymphocyte is High SCE(53.5SCE/cell), MMC sensitivity(-)(lymphocyte) | |
| Life Span | Crisis PDL | ||
| Morphology | fibroblast-like | Character | SV40 Ori-Transfection |
| Classify | transformed | Established by | Sasaki M. |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Methods for Passages | ||
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | Tissue Sampling | skin | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB3067 | Cell Name | AP39P(SVT) |
|---|---|---|---|
| LOT No. | 08122020 | Lot Specification | distribution |
| Medium | MEM alpha (GIBCO,Cat.No.12571-063) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML;Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-5.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.42x10^6 |
| Viability at cell freezing (%) | 99.07 | Antibiotics Used | free |
| Passage Number | p5 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:9,11 D13S317:10,12 D7S820:9,10 D16S539:10,12 VWA:16,17 TH01:6,9 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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