JCRB1821 BALM-27
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1821 | Cell Name | BALM-27 |
|---|---|---|---|
| Profile | B cell leukemia cell line, ALL-L3 cell line | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 67-year-old |
| Identity | not done | Tissue for Primary Cancer | peripheral blood |
| Case history | Acute lymphoblastic leukemia (ALL) | Metastasis | |
| Tissue Metastasized | Genetics | Karyotype information included in established papers | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphoblast-like | Character | Immnoglobulin phenotype and cell surface marker information are included in established paper. |
| Classify | tumor | Established by | Matsuo Y |
| Registered by | Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc | Regulation for Distribution | |
| Comment | Year | 2019 | |
| Medium | RPMI1640 medium with 10% featal bovine serum. | Methods for Passages | dilution (split ratio 1:2-1:3)/week |
| Cell Number on Passage | Race | Japanese | |
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:12389627 | A novel L3-acute lymphoblastic leukemia cell line (BALM-27) carrying cytoplasmic immunoglobulin 8 chain but lacking expression of cell surface immunoglobulin chains. Matsuo H,Drexler HG,Takeuchi M,Orita K Leuk Lymphoma. 2002 Jul;43(7):1441-6 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1821 | Cell Name | BALM-27 |
|---|---|---|---|
| LOT No. | 03132020 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO：Cat No.11875) with 20% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.5-1.0x10^6 cells/ml | Methods for Passages | 2-3x dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.0x10^7 |
| Viability at cell freezing (%) | 80.50 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:12 D13S317:8,12 D7S820:9,11 D16S539:9,10 VWA:17,20 TH01:7 AM:X TPOX:8,11 CSF1PO:12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | This cell line is very week for freeze and thawing.We recommend to start culture over 1x10^6 cells/ml. |
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