JCRB1818 Vero/TMPRSS2

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1818 Cell Name   Vero/TMPRSS2
Profile   Vero cells expressing the transmembrane serine protease TMPRSS2. Other Name   
Animal   monkey, African green Strain   
Genus    Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Takeda, M.
Registered by   Makoto Takeda Regulation for Distribution   
Comment    Year   
Medium   5% FBS-DMEM, Penicillin (100 unit/mL), Streptomycin (100 ug/mL), Geneticin (G418)(1mg/ml) Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_YQ48
Reference
Pubmed id:18562527Efficient multiplication of human metapneumovirus in Vero cells expressing the transmembrane serine protease TMPRSS2.
Shirogane Y,Takeda M,Iwasaki M,Ishiguro N,Takeuchi H,Nakatsu Y,Tahara M,Kikuta H,Yanagi Y
J Virol. 2008 Sep;82(17):8942-6

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1818 Cell Name   Vero/TMPRSS2
LOT No.   02072020 Lot Specification   distribution
Medium   DMEM(GIBCO:Cat No;11885-084) with 1 mg/ml G418(Thermo Fisher,Cat No;10131035) and 5% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.33x10^6
Viability at cell freezing (%)   97.65 Antibiotics Used   G418
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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