JCRB1742 Vn-324

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1742 Cell Name   Vn-324
Profile   Choroid plexus papilloma cell line of Syrian hamster induced by BK virus. Other Name   
Animal   hamster, Syrian Strain   Syrian
Genus   Mesocricetus Species   auratus
Sex    Age   
Identity   not done Tissue for Primary Cancer   brain
Case history   Choroid plexus papilloma induced by intracerebroventricular injection of BK virus. Metastasis   
Tissue Metastasized    Genetics   BK virus induced
Life Span   infinite Crisis PDL   
Morphology   polygonal-epithelial Character   
Classify   tumor Established by   Uchida S.
Registered by   Kousuke Kataoka Regulation for Distribution   
Comment    Year   2017
Medium   Dulbecco's modified Eagle's medium with 10% fetal calf serum Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA inPBS(-).
Cell Number on Passage   split ratio = 1/8 Race   
CO2 Conc.   5% Tissue Sampling   brain
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_VN84
Reference
Pubmed id:6264127Organization of viral genome in a T antigen-negative hamster tumor induced by human papovavirus BK.
Yogo Y,Furuno A,Nozawa A,Uchida S
J Virol. 1981 May;38(2):556-63

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1742 Cell Name   Vn-324
LOT No.   08012017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.97-1.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA,split ratio=1/8
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   94.4 Additional information   
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