JCRB3047 MK-19

Cell information

Cell type：human mutant cell repository from Kyoto University (View Pricing Information)

JCRB No.   JCRB3047 Cell Name   MK-19
Profile   Congenital anomaly (Mutant karyotype) 18-trisomy Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   
Case history   Congenital anomaly (Mutant karyotype) Metastasis   
Tissue Metastasized    Genetics   Trisomy 18
Life Span    Crisis PDL   
Morphology    Character   
Classify   primary Established by   SASAKI M.
Registered by   Radiation Biology Center, Kyoto University Regulation for Distribution   
Comment    Year   
Medium   RPMI1640 medium with 20% fetal bovine serum Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. (Split ratio=1/3)
Cell Number on Passage    Race   
CO2 Conc.    Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_LC38
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB3047 Cell Name   MK-19
LOT No.   08012017 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO) with 20% fetal bovine serum (SIGMA, Cat#172012, Lot#12J396, nonHI ) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.38x10^6
Viability at cell freezing (%)   95.6 Antibiotics Used   free
Passage Number   p7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,10
D7S820:8,11
D16S539:9
VWA:14,17
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Correct passage number is p7,but printed in the ampoule as p6
Cell No.   JCRB3047 Cell Name   MK-19
LOT No.   07272017 Lot Specification   distribution
Medium   RPMI1640 medium (GIBCO) with 20% fetal bovine serum (SIGMA, Cat#172012, Lot#12J396, nonHI ) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin (GIBCO) and 0.02% EDTA. (Split ratio=1/3)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.27x10^6
Viability at cell freezing (%)   87.7 Antibiotics Used   free
Passage Number   p8 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,10
D7S820:8,11
D16S539:9
VWA:14,17
TH01:6,9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   CELLBANKER1((Nihon Zenyaku Industries) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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