JCRB0152.4 M.P3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0152.4 Cell Name   M.P3
Profile   M cells derived from RLC-3 (JAR rat) was habituated to serum free culture medium. Other Name   
Animal   rat Strain   JAR-1
Genus   Rattus Species   norvegicus
Sex    Age   10-day-old
Identity   not done Tissue for Primary Cancer   
Case history   normal Metastasis   
Tissue Metastasized    Genetics   4-dimethylaminoazobenzene(DAB) transformed and remarkable changes were found in the biochemical properties.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Morphological changes observed cell line by DAB treatment. Historical cell line produced by Nagisa culture and DAB treatment.
Classify   transformed Established by   Katsuta,H. and Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment   Cells grow in serum free medium established by Takaoka,T. and Katsuta,H. Year   2015
Medium   DM201 medium. (This culture requires serum free medium definitely). Methods for Passages   Cells harvested by scraper and hard to individualize cells. Additional information is under preparation.
Cell Number on Passage   Split ratio = 1/2. Race   
CO2 Conc.    Tissue Sampling   liver
Tissue Type   tumor cells by DAB with Nagisa culture

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB0152.4 Cell Name   M.P3
LOT No.   03012017 Lot Specification   distribution
Medium   DM201 medium(Research Institute for the Functional peptides),Lipid and protein free condition. Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Harvest cells with scraper. 1/1-1/5 split
Doubling Time   NT Cell Number in Vial (cells/1ml)   NT
Viability at cell freezing (%)   NT Antibiotics Used   free
Passage Number   p27* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   FM-1 (Kyokuto Pharmaceutical Industrial Co.,Ltd.: #551-27100-3) and DM201 medium (1 to 1 mix) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mycoplasma has eliminated with MC-210(Final concentratre 0.5 ug/ml).
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