JCRB1721 NCMDF

Cell information

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JCRB1721 NCMDF細胞の分譲について

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1721 Cell Name   NCMDF
Profile   Normal Cynomolgus Monkey Dermal Fibroblasts Other Name   
Animal   monkey, Cynomologus Strain   
Genus   Macaca Species   fascicularis
Sex   M Age   21-month-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Normal Fibroblast
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   primary Established by   Matsuo, J.
Registered by   Matsuo, J. Regulation for Distribution   Restricted by Washington Treaty. End-user information is notified to the depositor.
Comment   The monkey that this cell line originated from was anti-herpes simian B virus (Macacine herpesvirus 1) antibody negative (tested by the establisher's organization). Year   2016
Medium   DMEM with 20% heat-inactivated fetal bovine serum Methods for Passages   0.25% Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   skin (abdomen)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_LC41
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1721 Cell Name   NCMDF
LOT No.   02142017 Lot Specification   distribution
Medium   DMEM(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.00-3.20x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.31x10^6
Viability at cell freezing (%)   99.70 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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Cell No.   JCRB1721 Cell Name   NCMDF
LOT No.   01152019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.3 - 4.5 x 10^4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 1.5 - 2 days Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   89.6 Additional information   
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