JCRB1716 Ex-3LL/CMV-Luc#1

Cell information

Important Notice(s)

Lewis lung carcinoma (3LL): Culture medium

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1716 Cell Name   Ex-3LL/CMV-Luc#1
Profile   Luciferase stably expressing cell line (Ex-3LL; Variant cell line possessing enhanced lung metastasis ability derived from Lewis lung cancer). Other Name   
Animal   mouse Strain   
Genus   Mus Species   musculus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   Yes
Tissue Metastasized   lung Genetics   pLVSIN-luc transfected Ex-3LL cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Lewis lung carcinoma derived cell line enhanced lung metastasis ability. Puromycin-resistant.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   Hams F10:L15=3:7 medium with 10% heat-inactivated fetal bovine serum. (Current medium for propagation; RPMI-1640 medium with 10% heat-inactivated fetal bovine serum.) Methods for Passages   Cells harvested after treatment with PBS containing 0.125% trypsin and 1 mM EDTA. Change medium twice a week.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG92
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LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1716 Cell Name   Ex-3LL/CMV-Luc#1
LOT No.   11292016 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.5-4.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.125%trypsin(GIBCO) and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   5.3x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
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Cell No.   JCRB1716 Cell Name   Ex-3LL/CMV-Luc#1
LOT No.   01202026 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; Nichirei Cat. # 174112, Lot 20M00K) Temperature   37 C
Cell Density at Seeding   approx. 8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.125% trypsin.
Doubling Time   approx. 1 day. Cell Number in Vial (cells/1ml)   3.6 x 10^6
Viability at cell freezing (%)   94 Antibiotics Used   free
Passage Number   Unknown (4 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI1640 CO2 Conc.   5%
Viability immediately after thawing (%)   91 Additional information   
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