JCRB1708 TYPK-2
Cell information
Important Notice(s)Announcement of unavailability of this cell line to overseas researcher outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1708 | Cell Name | TYPK-2 |
|---|---|---|---|
| Profile | Human pancreas adenocarcinoma cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 55-year-old |
| Identity | not done | Tissue for Primary Cancer | pancreas |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | resistant to oxaliplatin |
| Classify | tumor | Established by | Hirano, K. |
| Registered by | Regulation for Distribution | ||
| Comment | Year | 2015 | |
| Medium | Ham's F12 medium + DMEM (1 to 1 mix) with 5% fetal calf serum. | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | ascites |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:26124327 | Establishment and Characterization of Two Novel Human Pancreatic Carcinoma Cell Lines. Hirano K,Okumura T,Shimada Y,Watanabe T,Yamaguchi T,Nagata T,Tsukada K Anticancer Res. 2015 Jul;35(7):3821-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1708 | Cell Name | TYPK-2 |
|---|---|---|---|
| LOT No. | 04262017 | Lot Specification | distribution |
| Medium | DMEM and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.00-1.50x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.38x10^6 |
| Viability at cell freezing (%) | 99.43 | Antibiotics Used | free |
| Passage Number | p10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:8,13 D13S317:10,11 D7S820:10,12 D16S539:9 VWA:14,19 TH01:6,9 AM:X TPOX:9,11 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information | Mycoplasma was eliminated with Plasmocure. |
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