JCRB0144.2D LYM-1.P3D
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB0144.2D | Cell Name | LYM-1.P3D |
|---|---|---|---|
| Profile | Serum free FAR-2.F60 rat cell culture from lymph node near colon cancer. | Other Name | |
| Animal | rat | Strain | JAR-2 |
| Genus | Rattus | Species | norvegicus |
| Sex | Age | ||
| Identity | Tissue for Primary Cancer | ||
| Case history | tissue obtained from lymph node near colon cancer of the JAR-2 rat given MNU(0.4mg/ml). The MNU was applied to a bowel directly of two-week-old rat and tumors were found three mo. later. The lymph node was cultured by roller tubes with DM170 and 2% FBS. | Metastasis | |
| Tissue Metastasized | Genetics | Subclone of the LYM-1.P3. Morphologys and karyotypes were different from the LYM-1.P3B. Karyotype is lower diploid. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | Culture fruid of the LYM-1 strongly inhibited a growth of AH-7974 and other several cell lines. The inhibition was strongly suppressed by heating. |
| Classify | serum free | Established by | Kurayama,H. and Takaoka,T. |
| Registered by | Takaoka,T. | Regulation for Distribution | free |
| Comment | from LYM-1 | Year | 2015 |
| Medium | DM201 medium with serum free condition. | Methods for Passages | Cells harvested by gentle scraping. |
| Cell Number on Passage | 1.3 x 10^4 cells/sq.cm. | Race | |
| CO2 Conc. | Tissue Sampling | lymph node near colon cancer | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB0144.2D | Cell Name | LYM-1.P3D |
|---|---|---|---|
| LOT No. | 04132016 | Lot Specification | distribution |
| Medium | DM201 medium(Research Institute for the Functional peptides).Lipid and protein free condition. | Temperature | 37 C |
| Cell Density at Seeding | 2.5-3.27x10^4 cells/sq.cm | Methods for Passages | Cells were harvested with scraper. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.84x10^6 |
| Viability at cell freezing (%) | 95 | Antibiotics Used | free |
| Passage Number | p9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | FM-1 and DM201 medium(1/1) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 90 | Additional information |
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