JCRB1657 HuCCA-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1657 Cell Name   HuCCA-1
Profile   Human cell line derived from cholangiocarcinoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   54-year-old
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Sirisinha, S.
Registered by   Okada, S. Regulation for Distribution   
Comment    Year   2015
Medium   Ham's F-12 medium with 10% FBS Methods for Passages   0.5% Trypsin - 0.2% EDTA
Cell Number on Passage   Split ratio = 1/5 Race   
CO2 Conc.   5% Tissue Sampling   intrahepatic bile duct
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_M255
Reference
Pubmed id:1666951Establishment and characterization of a cholangiocarcinoma cell line from a Thai patient with intrahepatic bile duct cancer.
Sirisinha S,Tengchaisri T,Boonpucknavig S,Prempracha N,Ratanarapee S,Pausawasdi A
Asian Pac J Allergy Immunol. 1991 Dec;9(2):153-7




LOT Information

Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.
Cell No.   JCRB1657 Cell Name   HuCCA-1
LOT No.   02152017 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.6-5.6x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.5% trypsin and 0.02% EDTA.Split ratio=1/5
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.7x10^6
Viability at cell freezing (%)   98.00 Antibiotics Used   free
Passage Number   p14* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:8
D7S820:10,11
D16S539:11,12
VWA:14,19
TH01:9
AM:X
TPOX:9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Mycoplasma was eliminated with plasmocure.
Cell No.   JCRB1657 Cell Name   HuCCA-1
LOT No.   02052021 Lot Specification   distribution
Medium   Ham's F-12 medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   1.1 - 1.4 x 10^5 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 31 hrs. Cell Number in Vial (cells/1ml)   1.2 x 10^6
Viability at cell freezing (%)   99.4 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - F12 CO2 Conc.   5%
Viability immediately after thawing (%)   95.2 Additional information   
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