JCRB1646 HSC-3
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1646 | Cell Name | HSC-3 |
|---|---|---|---|
| Profile | Peripheral blood lymphocyte cell line of cynomolgus monkey (immortalized by Herpesvirus saimiri). | Other Name | |
| Animal | monkey, Cynomolgus | Strain | |
| Genus | Macaca | Species | fascicularis |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | transformed | Established by | Akari, H. |
| Registered by | Akari, H. | Regulation for Distribution | |
| Comment | Year | 2015 | |
| Medium | RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human). | Methods for Passages | Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood lymphocytes |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8610465 | In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses. Akari H,Mori K,Terao K,Otani I,Fukasawa M,Mukai R,Yoshikawa Y Virology. 1996 Apr 15;218(2):382-8 |
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LOT Information
Viability/Growth rate/Cell number are represented as actual values measured at lot presentation in JCRB, but are not guaranteed values. Additionally, the doubling time is a rough value measured during passages.| Cell No. | JCRB1646 | Cell Name | HSC-3 |
|---|---|---|---|
| LOT No. | 11102015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(50 U/ml)(Roche Interleukin-2,human) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-7.0x10^5 cells/ml | Methods for Passages | Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 6.34x10^6 |
| Viability at cell freezing (%) | 99.2 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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